Pannoramic SCAN II 2.2.0 User’s Guide
5.2.1 Profile Settings panel
Multilayer mode
•
Single layer mode:
If none of the filters are selected in the Filters table for multilayer
scanning, this mode is active and can be selected, and Multilayer mode turns inactive.
•
Extended focus:
By choosing the
Extended focus
option, the software selects the sharpest
image from each focus level for each image field, and combines them into one single image.
Therefore those parts that were blurry in auto/manual focus will also be sharp. This method
guarantees the maximum depth of sharpness, however, it reduces digitization speed.
•
Z-Stack:
For
Extended focus
and
Z-Stack
scanning methods, you can determine the desired
number of focus levels with the
Levels
selection box. The current focus value is the initial
level. If you set
5
for example, then the initial level, plus four (two focus levels above and
below the FOVs) are captured. Therefore, it is advised to choose an odd number for the focus
levels. Separate layers are created and stored this way, and can be opened with Viewer
applications. The Z-Stack slide contains the extended focus layers.
Step size
determines the spacing between focus levels. One step is around 0.2 µm. Default
settings are:
Levels
: 7;
Step size
: 5.
The number of iterations the Deconvolution algorithm is run can be determined in the
Iteration number for deconvolution:
value box.
NOTE:
The purpose of deconvolution is to produce a sharper image, but the use of a
deconvolution algorithm can significantly increase the time that takes to create a digital slide.
Figure 42 – Focus level and step size
January 24, 2020 - Rev. 2
3DHISTECH Ltd.
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