PANNORAMIC® SCAN RX 1.1
SCANNING MODES AND SETTINGS
NOVEMBER 18, 2021
63
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Figure 39. Focus level and step size
•
Z-Stack:
For
Extended focus
and
Z-Stack
scanning methods, you can determine the
desired number of focus levels with the
Levels
selection box. The current focus value is
the initial level. If you set
5
for example, then the initial level, plus four (two focus levels
above and below the FOVs) are captured. Therefore, it is advised to choose an odd
number for the focus levels. Separate layers are created and stored this way and can be
opened with Viewer applications. The Z-Stack slide contains the extended focus layers.
Step size
determines the spacing between focus levels. One step is around 0.2 µm.
Default settings are:
Levels
: 7;
Step size
: 5. The number of iterations the Deconvolution
algorithm is run can be determined in the
Iteration number for deconvolution
: value box.
Figure 40. Focus level settings
Notice:
The purpose of deconvolution is to produce a sharper image, but the use of a deconvolution
algorithm can significantly increase the time that takes to create a digital slide.
The capacity of the required memory for deconvolution is minimum 96GB RAM.
Scanning Settings
Includes the
Stop and go
scanning mode by which the slide tray stands still during recording the
FOV, then it moves on so that the scanner can record the next FOV.
