V9000T Trinocular Microscope
12
Using the Oil Objective (100x)
The procedure for examining a specimen using
an oil immersion objective is as follows:
Rotate the nosepiece so the low power objective
is in the optical path.
Place one drop of immersion oil on the lighted
area of the specimen slide
①
(Fig 12). Dust or
air bubbles in the oil can destroy the definition of
the image. If the bubbles are trapped between
the objective lens and the slide, clean off the oil
and start again or try to eliminate the bubble by
rotating the objective back and forth.
Rotate the nosepiece so the 100xR oil immersion
objective is in the light path.
With your eye at the level of the stage, use the
coarse focus knob to raise the stage with the
specimen cover glass. When you see a flash of
light at this location the objective lens has made
contact with the immersion oil and the
microscope can now be focused using the fine
focus knob.
Each time you finish using the oil immersion
objective wipe off all traces of oil from the
objective and the specimen cover glass with a
lens tissue or clean soft cloth. Cleaning after
each use will prevent oil from contaminating the
high dry objective (40xR) and deforming its
optical performance, prevent dust and dirt from
accumulating on the lens of the objective and
degrading its optical performance, and will keep
the slide clean to work with.
Fig. 12
①
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