Accu-Scope 3000-LED series Instructions Manual Download

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TROUBLESHOOTING GUIDE

PHASE CONTRAST MICROSCOPY

PROBLEM

CAUSE

CORRECTIVE MEASURE

Poor phase

contrast image

is obtained

The condenser phase annulus image
and the objective phase plate are not
aligned

Adjust the phase annulus so that it is
aligned with the objective phase
plate.

The condenser phase annulus and the
objective phase code do not match.

Rotate the phase annulus selector
wheel to the position that matches the
objective in use

The phase difference of the specimen
is too large.

Prepare the specimen using a
different refractive index immersion
fluid

The specimen cover glass is incorrect

Replace with 0.17mm thick cover
glass

Summary of Contents for 3000-LED series

Page 1: ...INSTRUCTIONS 73 Mall Drive Commack NY 11725 631 864 1000 P 631 543 8900 F www accu scope com info accu scope com 3000 LED SLIDER PHASE CONTRAST MICROSCOPY INSTRUCTIONS PHASE ANNULI ARE PRE CENTERED...

Page 2: ......

Page 3: ...technicians IMPORTANT Before you can use the phase slider you must remove the dust shield cover from the condenser housing and install the phase annuli slider in its place See FIG 1 IMPORTANT The cond...

Page 4: ...s recommended the iris diaphragm lever be set to PH on the condenser Insert the slider into the slot on the condenser as shown in FIG 3 FIG 4 Move the slider to its position that corresponds to the ph...

Page 5: ...rear focal plane The undiffracted light passes through this image It is reduced in intensity and given a one quarter wave phase shift by means of an annular phase pattern in the rear focal plane of t...

Page 6: ...nnulus so that it is aligned with the objective phase plate The condenser phase annulus and the objective phase code do not match Rotate the phase annulus selector wheel to the position that matches t...