Optimizing Performance
2
1100 Series WS MWS Reference Manual
45
Optimization for Lowest Carry-over
Several parts of an injection system can contribute to carry-over:
•
needle outside
•
needle inside
•
needle seat
•
sample loop
•
seat capillary
•
injection valve
The well plate sampler continuous flow-through design ensures that sample
loop, needle inside, seat capillary, and the mainpass of the injection valve is
always in the flow line. These parts are continuously flushed during an
isocratic and also during a gradient analysis. The residual amount of sample
remaining on the outside of the needle after injection may contribute to
carry-over in some instances. When using small injection volumes or when
injecting samples of low concentration immediately after samples of high
concentration, carry-over may become noticeable. Cleaning the needle in the
flush port or using the automated needle wash enables the carry-over to be
minimized and prevents also contamination of the needle seat.
Using the Automated Needle Wash
The automated needle wash can be programmed either as “injection with
needle wash” or the needle wash can be included into the injector program.
When the automated needle wash is used, the needle is moved into a wash vial
after the sample is drawn. By washing the needle after drawing a sample, the
sample is removed from the surface of the needle immediately.
Uncapped Wash Vial
For best results, the wash vial should contain solvent in which the sample
components are soluble, and the vial should
not
be capped. If the wash vial is
capped, small amounts of sample remain on the surface of the septum, which
may be carried on the needle to the next sample.
Summary of Contents for G1367A
Page 1: ...s1 Agilent 1100 Series Well plate Sampler Micro Well plate Sampler Reference Manual...
Page 4: ...4 1100 Series WS MWS Reference Manual...
Page 42: ...42 1100 Series WS MWS Reference Manual 1 Installing the Sampler...
Page 156: ...156 1100 Series WS MWS Reference Manual 4 Repairing the Sampler...
Page 196: ...196 1100 Series WS MWS Reference Manual 5 Parts and Materials...
Page 214: ...214 1100 Series WS MWS Reference Manual 6 Introduction to the Well plate Sampler...
Page 240: ...240 1100 Series WS MWS Reference Manual 7 Theory of Operation...
Page 276: ...276 1100 Series WS MWS Reference Manual 9 Specifications...
Page 284: ...284 1100 Series WS MWS Reference Manual Index...
Page 285: ......
