Allevi 3, User Manual

Page 1: ...1 Allevi 3 User Guide 1 0 3 ...

Page 2: ...2 BUILD W ITH LIF E ...

Page 3: ...STARTED 6 WHAT S IN THE BOX 7 ALLEVI 3 DIAGRAM 8 SETTING UP YOUR PRINTER 9 SETTING UP YOUR SOFTWARE 12 START PRINTING 13 ALLEVI SOFTWARE THE PROJECT WORKFLOW 14 PREPARING YOUR STL 15 BIOPRINTING IN THE DEVICE TAB 16 MAINTENANCE TROUBLESHOOTING 21 TROUBLESHOOTING AND CONTACT US 21 ...

Page 4: ...inks The second section of this guide will provide you with instructions on using the Allevi software to print a single material structure The third section will teach you how to print a multimaterial structure Finally the fourth section will teach you how to bioprint for your desired application Following this guide will help ensure that you are getting the most out of your machine We can t wait ...

Page 5: ...9 x 5cm 405 cm3 3 5 x 3 5 x 1 97 inch 24 1 in 3 Layer Resolution 100 microns Print File Type Gcode Extruder Max Temp 160 C Min Temp 4 C Pressure Range 0 120PSI 0 820kPa LED Range 365 nm or 405 nm WARNING The Allevi 3 includes moving parts that can cause injury Never reach inside the printer while it is in operation WARNING Do not leave the printer unattended while it is in operation WARNING Always...

Page 6: ...6 Let s Get Started ...

Page 7: ...7 WHAT S IN THE BOX Allevi 3 bioprinter Power cord Starter bioink kit Safety glasses Air compressor Compressor hose Air compressor arrives separately ...

Page 8: ...8 ALLEVI 3 DIAGRAM A Air Compressor Line B Swappable Extruder heating and cooling C Autocalibration pad D Build Plate E Power On Off ...

Page 9: ...er by connecting the compressor hose to nozzle on the left side of the printer labelled Step 4 This can be a tricky maneuver so for more in depth directions on connecting the air compressor watch this quick video The air compressor must remain turned on for the entirety of the print We recommend keeping the air compressor set to a pressure significantly higher than your print pressure This is so t...

Page 10: ...d requires no downloads We recommend Google Chrome or Firefox but any of the following browsers are also acceptable Safari Opera Edge or Internet Explorer 2 To set up an account fill out the form and click Register 3 To register your printer go to http bioprintallevi com devices Click Register ...

Page 11: ... in your device number and click Register again Your device number can be found in your onboarding email sent to you by the Allevi Support Team 5 Once your printer is registered it will appear as an available printer ...

Page 12: ...nnect your printer to your WiFi network or connect to the Offline Interface a To connect to WiFi choose your WiFi network from the dropdown menu under SSID type in the password and hit connect The chosen WiFi network should be a private network Your computer s WiFi will be restored and your printer will connect to the Internet b To connect to the Offline Interface click on the button on the righth...

Page 13: ...13 Let s Start Printing ...

Page 14: ...es the various projects you re working on and makes them easily accessible In a project you can store data notes pictures and prints The Allevi software also hosts other projects from Allevi and other community members You can copy a public project into your explorer allowing you to replicate and modify others studies ...

Page 15: ...egory of Build With Life There are also many 3D models available on the internet To begin we will use a predesigned STL file The Allevi software project workflow incorporates a built in slicer However for now we will use Repetier Host as our slicing software Repetier Host is a third party software that converts STL files 3D models into gcode files instruction the printer can read You can find the ...

Page 16: ...f and it s connected 2 Login to bioprintallevi com and go to the Devices tab Select your printer which should be already registered and click Connect 3 Load your syringe with Pluronic F127 Visit the Allevi Protocols page to watch a video on how to load syringes for your Allevi 3 We suggest filling your syringe with about 3 ml of material for your first print a Attach a 30 gauge 0 25 pink needle to...

Page 17: ...d your extruder you can test its extrusion move it around in the Jog Panel or calibrate it a For autocalibration click the Autocalibrate button Autocalibration takes 2 minutes and will avoid the need for the Jog Panel For short needles only add the wellplate after autocalibration is complete otherwise the extruder will crash ...

Page 18: ...commend using the 20mm step size iii Switch step size from 20mm to a smaller size depending on the needle length Then use up and down buttons to move the bed plate Z closer to the extruder to the point where the dish is almost touching the needle Do not leave a big gap between the needle and dish as the gcode automatically adds the appropriate layer height Finer 0 1mm movement can be used when app...

Page 19: ...ng the UV Visible toggle There are three main settings for crosslinking Always On During Print and Post Print They use variables such as light intensity layer frequency and duration 6 Click the Upload button to upload a gcode file from your computer It will render a rotatable 3D visualization of the extruder path ...

Page 20: ...llplate calibration and gcode file are all ready you can press Start Print to start building with life a Note As the printer loads the gcode the printer s yellow lights will turn on 8 Sit back and watch your printer go ...

Page 21: ...he experience and expertise Allevi has to offer One very useful resource is Allevi s Build With Life repository We upload protocols experiments and guides to help you be more successful with bioprinting You can also visit the Allevi YouTube channel for video instructions Have more questions or need help Visit us on the Allevi Support page or email us at support allevi3d com ...

Page 22: ...22 Allevi Inc 3401 Grays Ferry Ave Building 176 Philadelphia PA 19146 Copyright Allevi Inc 2018 ...

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