6.
Turn on illumination with the
LIGHT ON
Button
located on the left side of the
control bar.
7.
Focus the sample with
focusing knobs
.
8.
Optional:
To take a picture of the
transmitted light image, click the
Capture
Button
on the control bar.
9.
Place the
light shield box
on the stage,
over the sample.
10.
Move the
light cube selection lever
to the desired fluorescence channel
(the
Channel Bar will highlight the selected light
cube).
11.
With the
Find & Focus Tab
active, turn on
fluorescence illumination using the
LIGHT
ON Button
.
12.
Adjust the focus as necessary.
13.
Adjust the
Illumination Intensity Slider
on
the control bar as needed.
14.
Click the
Capture Button
.
15.
Repeat steps 10–14 to acquire each
fluorescence channel.
16.
Click the
Overlay Tab
to show all channels
in color overlay mode.
17.
Adjust
Brightness and Contrast
for each
channel to bring them into desired balance.
18.
Click the
Save Button
to save the color
image
(refer to EVOS fl User Guide)
.
3
Brightness and Contrast
Overlay Tab
Save Button
LIGHT ON Button
Capture Button
Illumination Slider
