1.
Channel Bar
(active channel highlighted)
2.
Mode Tabs
(Find & Focus, Actual, Overlay)
3.
LIGHT ON/OFF Button
4.
Illumination Intensity Slider
5.
Capture Button
6.
Boost and Color Options
7.
Settings Tab
8.
Save Button
9.
Information Bar
10.
Selected Objective
4
Helpful Tips
Faint signals near background can be
amplified by turning on the
Boost Option
.
This only changes the way the image is
displayed; it does not alter the data.
Boost and Color Options
In Find & Focus or Actual Mode, the
Color Option
can be turned off to display a grayscale image. This
often shows more details than a color image.
In Find & Focus Mode, the exposure time is set to 100 ms to assist real-time focusing, moving the
stage, etc. The illumination level is approximately 60% of the amount used for image capture, in order
to minimize photobleaching and phototoxicity. Clicking
Capture
results in brighter illumination and a
longer exposure time during image capture to provide a high-quality image.
In Actual Mode, turning on the illumination results in full-powered illumination and actual exposure
times for live viewing of the sample. With longer exposure times
(more than 200 ms)
there will be a lag
between moving the focus knob and seeing the focus change onscreen.
Note:
The EVOS fl User Guide is on the USB flash drive in the accessories box. You can also download it
from the EVOS fl
product page at
www.amgmicro.com
.
