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Copyright © 2010 Advanced Microscopy Group. All rights reserved.

ZP-PKGA-0494 REV A

Summary of Contents for EVOS fl

Page 1: ...ZP PKGA 0494 REV A amgmicro com QUICK START GUIDE Digital Inverted Microscope for Fluorescence and Transmitted Light Applications ...

Page 2: ...beam and use protective shields NEVER look directly at UV light Basic Operation 1 Turn on the microscope with the power switch on the right side of the base 2 Plug a USB flash drive into one of the USB ports on the right side of the microscope 3 Place the sample on the stage using a vessel holder if needed 4 Set magnification with the objective selection wheel on the front of the microscope 5 Pull...

Page 3: ...be 11 With the Find Focus Tab active turn on fluorescence illumination using the LIGHT ON Button 12 Adjust the focus as necessary 13 Adjust the Illumination Intensity Slider on the control bar as needed 14 Click the Capture Button 15 Repeat steps 10 14 to acquire each fluorescence channel 16 Click the Overlay Tab to show all channels in color overlay mode 17 Adjust Brightness and Contrast for each...

Page 4: ...Find Focus Mode the exposure time is set to 100 ms to assist real time focusing moving the stage etc The illumination level is approximately 60 of the amount used for image capture in order to minimize photobleaching and phototoxicity Clicking Capture results in brighter illumination and a longer exposure time during image capture to provide a high quality image In Actual Mode turning on the illum...

Page 5: ...ut jack USB and DVI ports Coarse stage positioning knobs Stage X axis knob Stage Y axis knobs Focusing knob Phase annuli selector Condenser slider slot Objective selection wheel 5 Light cube selection lever left side of base ...

Page 6: ...Copyright 2010 Advanced Microscopy Group All rights reserved ZP PKGA 0494 REV A ...

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