Andor Technology Optosplit II, User Manual

Page 1: ...Optosplit II Image Splitter User Guide v3 1 ...

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Page 3: ... co uk The following points should be considered when using the Optosplit for the first time 1 The Optosplit is supplied pre aligned and centred please refer to this manual before making any adjustments Do not adjust smaller knurled screws or recessed hex screw on lid before reading this manual 2 When changing the filter cube care should be taken not to touch any optical surfaces If any lenses fil...

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Page 5: ...ith female c mount input Output focussing adjuster with male c mount output Filters Dichroic filter ____________ Short emitter ____________ Long emitter ____________ Dichroic filter ____________ Short emitter ____________ Long emitter ____________ Dichroic filter ____________ Short emitter ____________ Long emitter ____________ Dichroic filter ____________ Short emitter ____________ Long emitter _...

Page 6: ...troduction 1 2 Installation guide 3 2 1 Configuring the filter and dichroic mirror holder 4 2 2 Installing the Optosplit in the light path 5 2 3 Focussing the camera 6 3 Operation of the Optosplit 7 3 1 Adjusting the position of the images 7 3 2 Configuring a new filter set 9 4 Technical summary 11 5 Technical Support 13 ...

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Page 9: ...Cairn Optosplit allows the researcher to do exactly this The Optosplit is usually supplied with a rectangular aperture to define an ROI and includes controls to allow the two images to be positioned accurately and conveniently within the camera frame Images can be acquired using any imaging software and processed either manually off line or using an appropriate analysis tool such as the Splitview ...

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Page 11: ...is available Figure 1 Optosplit with adjustable rectangular diaphragm Before installing the Optosplit you will first need to install an appropriate filter cube into the filter holder If your Optosplit has been purchased with a full filter set pre installed then you can skip to section 2 2 for instructions on fitting it in the optical pathway Instructions for installing the filter cube are given in...

Page 12: ...is with the arrow pointing towards the sample i e Towards the microscope The filters are held in place using the 25mm loking rings supplied and the dichroics accessed by removing the four screws holding the two halves of the cube together The filter cube is fitted to the runner indicated in figure 3 below Figure 3 Fixing the filters in position Note When fixed correctly in the cube the filters sit...

Page 13: ...e Once a clear image can be seen the camera should be switched off and removed from the microscope The Optosplit should be fitted onto the microscope with the aperture orientated towards the C mount port The CCD camera can then be fitted onto the exit port of the Optosplit The exit port of the Optosplit has a rotating ring green arrow in fig 6 to allow the camera to be fitted in the same orientati...

Page 14: ...two hex screws that retain the output rotating ring green arrow to allow the camera to turn in the C mount 3 Separate the two images slightly using the knurled screw facing towards the camera blue arrow 4 Slightly loosen the single hex screw which locks the focussing ring red arrow 5 Rotate the focussing ring between the camera and the Optosplit whilst holding the Optosplit and camera in position ...

Page 15: ...ase note that the diagrams below indicate the images for a system using an appropriately aligned and centred rectangular diaphragm if your system has an iris diaphragm then the images will be shaped accordingly An image similar to that shown below will be observed when the Optosplit is first used with the two images superimposed Figure 8 Superimposed images on aligned system When the camera is mou...

Page 16: ...rtical heights this can be corrected by adjusting the position of the short wavelength image Turning the vertical alignment adjustment Pre 1 will alter the vertical position of the shorter wavelength image Again only very small adjustments are necessary 3 Ready to record Figure 11 Correct alignment Once the spectrally resolved images are located side by side on the camera chip you are ready to rec...

Page 17: ...2 Centre the same image vertically using the vertical offset adjustment on the top plate of the Optosplit Pre 1 3 The short wavelength image is now correctly positioned 4 To centre the long wavelength pathway we superimpose the longer wavelength image on the short wavelength image we have just centred This is done using the knurled screw Pre 3 and hex screw Pre 4 5 First we centre the image horizo...

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Page 19: ...radle to give precise symmetrical control of the degree of separation whilst maintaining identical path lengths A half size fully silvered mirror at the output port for recombining the images Fine adjustment controls for camera focus and vertical alignment Optional ROI definition using adjustable rectangular aperture or iris diaphragm The system is supplied with high grade AR coated achromatic dou...

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Page 21: ...port 5 Technical Support e mail tech cairn research co uk Web http www cairn research co uk Address Cairn Research Ltd Graveney Road Faversham Kent ME13 8UP Telephone 44 0 1795 590 140 Fax 44 0 1795 594 510 Page 13 ...

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