Ankom XT10, Operator'S Manual

Operator’s

Manual

pg.

10

Rev

E

4/2/12

Fat

Extraction

Process

Crude

Fat

Calculation

Crude Fat contained within a food or feed sample can be calculated using the following formula: 

%

Crude

Fat

=

100

x

(W

2

–

W

3

)

W

1

Where:

W

1

=

Original

weight

of

sample

W

2

=

Weight

of

pre

‐

dried

sample

and

filter

bag

W

3

=

Weight

of

dried

sample

and

filter

bag after

extraction

For meat samples with > 15% fat and plant samples with > 20% fat: 

%

Crude

Fat

=

100

x

((W

2

–

W

4

)

–

W

3

)

W

1

Where:

W

4

=

Weight

of

the

weigh

tin

Total

Fat

Calculation

If hydrolysis is done prior to fat extraction, then Total Fat contained within a food or feed sample can be calculated 
using the following formula: 

%

Total

Fat

=

100

x

(W

2

–

(W

3

+

(C

1

–

C

2

)))

W

1

Where:

W

1

=

Original

weight

of

sample

W

2

=

Weight

of

dried

sample,

filter

bag,

and

Diatomaceous

Earth

(DE)

after

hydrolysis

W

3

=

Weight

of

dry

extracted

sample,

filter

bag,

and

DE

C

1

=

Blank

filter

bag

dry

weight

after

hydrolysis

C

2

=

Blank

filter

bag

weight

after

extraction

Fat

Extraction

Support

Items

The following support items are needed to perform the fat extraction procedure: 

Item

Recommended

Product

Electronic Balance with four-place readout

ANKOM #TB Balance Hardware 
ANKOM #TBS Balance Software 

Filter Bags

ANKOM #XT4

Bag Holder (used for adding sample to an empty filter bag)

ANKOM #101.2

Heat Sealer for sealing the filter bags

ANKOM #1915 (120V), #1920 (220V)

Solvent Resistant Marker

ANKOM #F08

Desiccant Pouch or Weigh Tin Desiccator for drying

ANKOM #X45, #X49

Oven for drying (capable of maintaining 102°C±2°)

ANKOM #RD (120V), #RDI (220V)

Ventilated oven trays to allow for sufficient oven air flow 

----------------- 

Weigh Tins (used with samples of > 20% fat)

----------------- 

Sample 

----------------- 

Spoon

-----------------