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2022/03/16 04:45

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MS2000, MFC2000 and RM2000 OPERATION

Applied Scientific Instrumentation - https://asiimaging.com/docs/

transitions can be counted to provide a “pixel” clock for an external recording device.

Synchronous Encoder Reporting

The 

TTL_REPORT_INT

 firmware module allow for external TTL synchronized position reporting. The

position reports are sent to the auxiliary serial port on the MS 2000 WK in a binary format so that
rapid, low jitter position reporting is possible for real-time positioning tasks. Contact ASI for details.

Tracking Features

ASI’s PhotoTrack system uses the 

TRACKING

 firmware module along with hardware available from ASI

that allows the stage to latch on and track spots of fluorescent illumination from labeled organisms. A
quadrant PMT is used to provide sensitive and rapid position feed back information from the
illuminated target. Contact ASI for details.

Safety, Diagnostic and Alignment Features

Motor-driver current-limits prevent the motors from fully powering into hard stops. In addition, the MS
2000 controller is constantly monitoring the positions of the motors under its control. Situations that
may result in run-away conditions (such as reversed polarity motors or encoders), or situations where
the motor is not able to follow the desired move trajectory (e.g., when mechanical interference limits
the motion), cause the controller to detect an error condition. The motors are immediately turned off
and the offending axis is disabled. If this happens, a ‘D’ character is displayed as the status indicator
on the LCD display. The user should correct the problem and then reset the controller to regain
control of the disabled axis.

Controllers using firmware Version 6.0 and newer utilize a motor driver circuit where all analog circuit
alignment is done either automatically in firmware, or via serial commands. The user need not open
the case to adjust the drive-circuit feedback and zeroing levels.

The firmware also keeps track of any internal error conditions that may arise during operation and
saves the last 255 error codes in a buffer that may be read out for diagnostic purposes (see the serial
DUMP command). The controller also has a built in “move buffer” that holds move dynamic
information for up to 200 servo cycles. The user may utilize this buffer to attempt advanced tuning of
the controller for special applications or extremely fast or slow moves. Please see the section MS
2000 Optimal Alignment Procedures for a full discussion of these issues.

Clocked Devices

The MS 2000 controller supports clocked rotational devices, such as motorized objective nosepiece
turrets, filter cube turrets, and filter wheels. These devices move to discrete clocked positions. Manual
control is usually via the @ button to advance to the next position. The serial MOVE command is used
to move the devices to a specific integer-value clocked position. The current position is reported using
the WHERE command.

Summary of Contents for MFC2000

Page 1: ...ings for the joystick may be programmed and saved in rmware See the JSSPD command Command Encoder Knob The Command Encoder Knob is usually used to control the Z axis stage The relative speed of the kn...

Page 2: ...witch is not used or used for other special purposes LCD Screen Note MS2000 and RM2000 have a 4 line LCD MFC2000 controllers only have a two line LCD The Liquid Crystal Display LCD screen shows the cu...

Page 3: ...ell On the right side is a time clock Some error codes are displayed in place of the clock for a few seconds after they occur Mode 3 SW 1 2 UP Dual Display with Status Line Shown 4 line version 2 line...

Page 4: ...til the axis reaches target to the accuracy speci ed by the PC error variable Should the stage drift further from the current target by more than the E drift error variable the motors will re engage a...

Page 5: ...port is controlled by a second UART on the microcontroller As a default it is con gured as a pass though so serial tra c sent to the controller from the PC is echoed directly on the OUT port Special f...

Page 6: ...ces Switches 1 2 select the LCD screen options Switches 4 and 5 set up the serial baud rate for the RS 232 and USB interfaces Switches 3 6 select between linear and rotary encoders for the XY and Z ax...

Page 7: ...ten there are special rmware modules that are included to provide custom functionality The BUILD X BU X command lists the rmware basic build avor and all of the special rmware modules that are include...

Page 8: ...the current stage position will be lost Upon loss of power the controller will send the character O out the serial port After successfully saving positions the controller will send the character K Sav...

Page 9: ...near encoded stages and especially when a second axis remains in motion after one axis has nished moving Turning the drivers o and using error threshold hysteresis means that most of the time there is...

Page 10: ...teger multiple of the slowest speed Be aware that at the very slowest speeds the condition and cleanliness of the stage and the calibration of the analog stage driver circuitry can have a dramatic e e...

Page 11: ...ontroller to detect an error condition The motors are immediately turned o and the o ending axis is disabled If this happens a D character is displayed as the status indicator on the LCD display The u...

Page 12: ...some examples of the con gurations settings that can be changed See the CCA command for details The con guration ags are usually only changed when the controller is rst set up for a particular set of...

Page 13: ...king memory and clears the data from the Saved Position nonvolatile memory locations to ready those storage locations for when power is again shut o If the controller is RESET without turning o the po...

Page 14: ...tems On systems with a PIEZO axis this is the controled voltage when a commanded move to the PIEZO usually Z axis is made DAC_OUT with WRDAC command provides external analog output 6 TTL IN1 INPUT Aux...

Page 15: ...ted 9 N C Not Connected 10 X Lim U X Upper Limit 11 5V 5V X limits 12 Y Lim U Y Upper Limit 13 5V 5V Y limits 14 X Mot X Motor 15 5V 5V X encoder 16 X Enc Ch B X Encoder Channel B 17 Y Mot Y Motor 18...

Page 16: ...TION 2 R In Receive 3 T Out Transmit 5 GND Signal Ground 1 4 6 9 N C Not Connected RS 232 Serial Out DB 9M Connector PIN SIGNAL INFORMATION 2 T Out Transmit 3 R In Receive 5 GND Signal Ground 1 4 6 9...

Page 17: ...Connected IN BNC optional PIN SIGNAL INFORMATION Center TTL IN VIH 3 2V VIL 1 3V Outer GND Signal Ground OUT BNC optional TTL PIN SIGNAL INFORMATION Center TTL OUT VOH 4 4V VOL 0 1V IO Max 50mA Outer...

Page 18: ...0 10v 1 If you do not see Ports COM LPT when the drivers are installed and the controller connected and powered up then the computer may not fully support USB and RS 232 Certain inexpensive laptops ha...

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