Operation
•
13
3. In
the
Filter Tool
program, click the checkbox for the filter position you are
adding.
4. Enter the midpoint of the wavelength range that is transmitted by the filter.
For example, a 530DF30 filter transmits a 30 nm band of light centered at
530 nm (
i.e,.
it transmits 515 – 545 nm), therefore the midpoint of this filter
is entered as “530”. For a long-pass filter, enter the peak emission of the
dye you will use with that filter.
5. In GenePix Pro you can display either the filter’s
Formal name
(
e.g.,
“530DF30”) or an
Alternate name
(
e.g.,
“Alexa 488” or “Blue Filter”).
Enter both names here so that each user can choose their preferred
nomenclature in GenePix Pro.
6. After you have entered this information for each new filter, click
Save
.
7. Close
the
Filter Tool
program.
When you open GenePix Pro, the new filters are listed in the Hardware Settings
dialog box.
Chapter 3
