BD Accuri C6, Instrument Manual

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Page 3: ...8 3 3 2 Connecting the Cables 9 3 4 Installing Software 10 3 4 1 Installing BD Accuri C6 Software 10 3 5 Starting Up the BD Accuri C6 Flow Cytometer 10 3 6 Turning Off the BD Accuri C6 Flow Cytometer...

Page 4: ...3 8 Improper Shut down Message 11 Table 4 1 BD Accuri C6 Flow Cytometer Replacement Parts Reagents List 13 Figure 4 1 Automated Preventive Maintenance Notification Window 14 Figure 4 2 Waste Line Quic...

Page 5: ...pecifications setup and maintenance It also includes common troubleshooting help and service information For information on using BD Accuri C6 Software see the BD Accuri C6 Software User Guide For inf...

Page 6: ...ates 10 100 L min Custom Core Diameter 5 40 m Recommended Sheath Fluid 0 22 m filtered deionized DI water Maximum Events Per Sample 1 million events per well Florescence Sensitivity MESF FITC 150 PE 1...

Page 7: ...ent or computer Clean up spills immediately Never place anything on top of the cytometer including sample tubes and racks Turn off the instrument and unplug the power cord before manually cleaning the...

Page 8: ...01 Rev 2 To obtain an MSDS visit www AccuriCytometers com or contact In the USA Email TechSupport AccuriCytometers com Call Accuri Technical Support at 1 734 994 8000 Outside the USA Visit www Accuri...

Page 9: ...The following figures show the major components of the fluidics and optics subsystems Figure 3 1 BD Accuri C6 Flow Cytometer Optics and Fluidics Top View Lid Open Set up the computer monitor keyboard...

Page 10: ...change the Put the computer to sleep setting to Never using the drop down function we also suggest setting the Turn off the display setting to Never d Select Save Changes Then close the control panel...

Page 11: ...stalled see section 4 11 for instructions on replacing the filter Figure 3 2 In line Sheath Filter 4 Verify that there are no disconnected electronic cables on the optics assembly 5 Verify that the in...

Page 12: ...rate PN 653157 solutions as instructed and fill decontamination and cleaner bottles respectively Close the bottles securely Do not over tighten 3 Add approximately 100 mL of 0 5 NaOCl to the Waste bot...

Page 13: ...rd outlet Only operate this instrument from a supply source that incorporates a third wire protective grounding conductor A surge suppressor or power strip is recommended 3 Connect the USB cable to th...

Page 14: ...izard 1 Check fluid levels in all bottles ensuring that the Waste is empty and the Sheath Cleaner and Decontamination bottles are full 2 Gently push the sample stage back remove and discard the SIP co...

Page 15: ...dation beads provided with the cytometer 1 Place a tube with 2 mL of decontamination solution on the SIP 2 Select an empty data well in the Collect Tab of BD Accuri C6 Software 3 Set the time limit fo...

Page 16: ...BD Accuri C6 Flow Cytometer Instrument Manual 12 7820018 01 Rev 2 NOTE It is not necessary to exit BD Accuri C6 Software or turn off the computer before turning off the cytometer...

Page 17: ...154 Decontamination Concentrate Solution makes 200 mL 653155 Decontamination Concentrate Solution makes 1 L 653156 Bacteriostatic Concentrate Solution for treating 10 L Sheath Fluid 10 vials 653157 Cl...

Page 18: ...ing Remind me later will set the notification to remind the user in 5 days Figure 4 1 Automated Preventive Maintenance Notification Window Run the backflush cycle as needed to clean the SIP and remove...

Page 19: ...th a tube of 2 mL 0 22 m filtered DI water 5 Restart the cytometer The cytometer performs a longer fluidics startup cycle and the Software displays the message Extra startup time needed due to cleanin...

Page 20: ...complete 4 Set the run time in the software control panel to five minutes and click on the Run button Allow the cytometer to run until it stops automatically NOTE The peristaltic pumps will make a rhy...

Page 21: ...s a message when the fluid bottles need attention or if a bottle level sensor is improperly connected Each of the sheath cleaner and decontamination bottles contain a disk filter Replace these filters...

Page 22: ...ne should be considered a biological hazard Do not attempt to clean up the fluid without gloves and other safety attire Dispose of all cleaning materials as if biologically contaminated Replace the in...

Page 23: ...ical samples and therefore should be considered hazardous Wear gloves and appropriate safety gear during this procedure There are two peristaltic pumps a sheath pump and a waste pump 1 Turn off and un...

Page 24: ...Replace with new peristaltic pump tubing PN 653146 by sliding the luer lock fittings on the pump head 8 Snap the pump element retainer clip into place Reconnect the luer lock fittings on the outside o...

Page 25: ...re See the BD Accuri C6 Software User s Guide or Quick Start Guide Collection for instructions on using BD Accuri C6 Software Connect the USB cable from the C6 to one of the computer USB ports CAUTION...

Page 26: ...am Files BD Accuri BD Accuri C6 Software libraries Then click on the OK button to enter the selected path into the Browse window Figure 5 1 Browse For Folder Dialog Box Click the Next button Figure 5...

Page 27: ...e driver software When prompted click the Close button Figure 5 4 Update Driver Software Cytometer Successfully updated your driver software With the C6 driver installed and the USB cable properly con...

Page 28: ...3 Run a backflush to see if the SIP is clogged see section 4 2 4 Make sure the peristaltic pump tubing and retainer clips are properly attached see section 4 12 5 Restart BD Accuri C6 Software 1 Check...

Page 29: ...power supply in the Pelican Case When disconnecting the fluidic harness from the cytometer always disconnect from the bottle end of the harness first to prevent fluid leaks NOTE Contact Accuri Techni...

Page 30: ...echnical Support techsupport europe bd com Canada BD Biosciences 2100 Derry Rd West Suite 100 Mississauga Ontario Canada L5N 0B3 Email canada bd com Direct Phone Numbers Austria 0810 101 807 Belgium 0...

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