User’s Guide
22
System status is also displayed on the Status screen. See
for a description of the Status screen.
•
Activity.
Shows whether the cytometer power is on and the
status of acquisition. The following table describes the
indicator LEDs, and the status that triggers them.
Fluid control
The three fluid control buttons (Run, Standby, and Prime) set the
cytometer operation.
•
Run.
Pressurizes the sample tube to transport the sample
through the sample injection tube and into the flow cell.
The Run button is green when the sample tube is on and the
support arm is centered. When the tube support arm is moved
left or right to remove a sample tube, the cytometer switches to
an automatic standby status to conserve sheath fluid, and the
Run button changes to orange.
•
Standby.
Stops fluid flow to conserve sheath fluid.
When you leave the cytometer for more than a few minutes,
place a tube containing 1 mL of deionized (DI) water on the
sample injection port (SIP) and press
Standby
.
•
Prime.
Prepares the fluidics system by draining and filling the
flow cell with sheath fluid.
The fluid flow initially stops, and pressure is reversed to force
fluid out of the flow cell and into the waste container. After a
preset time, the flow cell fills with sheath fluid at a controlled
rate to prevent bubble formation or entrapment. At
completion, the cytometer switches to standby mode.
Indicator
LED color
Status
Steady pulse
Blue
Cytometer is powered on.
Fluctuates
Blue
Cells are passing through the
flow cell.
Summary of Contents for FACSymphony A3
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Page 97: ...Chapter 6 Recording and analyzing data 97 e Click OK 12 Print the analysis...
Page 98: ...User s Guide 98 Your global worksheet analysis objects should look like the following...
