Beckman Coulter Optima LE-80K, Instruction Manual

Page 1: ...Published by the Centrifuge Instrument Systems Development Center of Beckman Coulter Inc Palo Alto California 94304 LLE7 8 IM 5 Instruction Manual Optima LE 80K Preparative Ultracentrifuge TM ...

Page 2: ...on consulter les documents joints Atención consulte los documentos adjuntos Attenzione consultare le informazioni allegate On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione Off power Aus Netzverbindung Arrêt mise hors tension Apagado Spento fuori tensione Protective earth ground Schutzleiteranschluß Liaison à la terre Puesta a tierra de protección Collegamento di...

Page 3: ...ll and relevel the instrument in its new location Any servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury Make sure that the power switch is turned off and the instrument is disconnected from the main power source and refer such servicing to qualified personnel Electrical Safety To reduce the risk of electrical...

Page 4: ... transmit disease No known test offers complete assurance that they are free of micro organisms some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacterium and certain systemic fungi further emphasize the need for aerosol protection Handle other infec tious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generat...

Page 5: ...fuge Instrument Systems Development Center of Beckman Coulter Inc Palo Alto California 94304 LLE7 8 IM 5 January 2003 Instruction Manual Optima LE 80K Preparative Ultracentrifuge 2003 Beckman Coulter Inc Printed in the U S A ...

Page 6: ...I S EN ISO 9001 ...

Page 7: ...andby Mode 1 1 Control Panel 1 1 Hardkeys 1 2 Displays 1 2 Run Status Indicators 1 3 Diagnostic Messages 1 3 Door 1 4 Rotor Chamber 1 4 Vacuum System 1 5 Temperature Sensing 1 6 Temperature Control System 1 6 Overspeed System 1 6 Rotor Energy System 1 7 Drive 1 7 Name Rating Plate 1 7 SECTION 2 RUN PREPARATION Entering Information 2 1 Standard Operation 2 2 Keypad 2 2 _ a Clear Entry 2 2 2 2 ENTER...

Page 8: ...nction 2 9 Entering Program 0 2 10 Running Program 0 with a Second Program 2 10 SECTION 3 RUN PROCEDURE Standard Operation 3 2 Programmed Operation 3 2 Delayed Start Program Function 3 3 Points to Remember 3 3 SECTION 4 TROUBLESHOOTING AND MAINTENANCE In Case of Power Failure During the Run 4 1 Retrieving Your Sample 4 2 Diagnostic Messages 4 6 Cleaning 4 7 Rotor Chamber 4 7 Decontamination 4 9 St...

Page 9: ...r 4 3 Figure 4 2 Depressing the Latch to Loosen the Front Panel 4 4 Figure 4 3 Interior View of Instrument 4 5 Figure 4 4 Reinstalling the Front Panel 4 6 Figure 4 5 Location of Diagnostic Messages on the Control Panel 4 7 Page Table 1 1 Run Status Indicators 1 4 Table 2 1 Vacuum LEDs 2 8 Table 4 1 Diagnostic Troubleshooting Chart 4 8 ...

Page 10: ...operation and routine operator care and maintenance The following introductory pages contain the instrument specifica tions and preinstallation requirements Section 1 provides a brief description of the instrument the operating controls and indicators Section 2 explains how to use the control panel to enter run parameters for standard and programmed operation Section 3 covers operating procedures f...

Page 11: ...ertain symbols are used throughout this manual to call attention to safety related and other important information The symbols used and their definitions are as follows NOTE Used to call attention to information that should be followed during installation use and or servicing of the equipment CAUTION Used to indicate a potentially hazardous situa tion which if not avoided may result in minor or mod...

Page 12: ...TERFERENCE This instrument has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environ ment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordanc...

Page 13: ... set out in the radio interfer ence regulations of the Canadian Department of Communications Le présent appareil numérique n émet pas de bruits radioélectriques dépassant les limites applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage radioelectrique édicté par le Ministère des Communications du Canada ...

Page 14: ...m at speed above 1000 rpm Time Set time up to 99 hours and 59 minutes HOLD for runs of unspecified length Time display indicates time remaining in timed runs time elapsed in HOLD runs or estimated time remaining in ω2 t runs Rotor Temperature Set temperature 0 to 40 C in increments of 1 C Temperature control 1 C of set temperature Temperature display indicates rotor temperature in increments of 0 ...

Page 15: ...unter displays accumulated total number of rotor revolutions in tens of thousands Barrier Ring 41 mm 1 63 in heat treated steel alloy armor ring surrounded by a 12 mm 0 50 in steel vacuum chamber to provide full protection for the operator Door 17 5 mm 0 69 in high strength structural steel Vacuum diffusion pump in series with a mechanical pump reduces chamber pressure to below 5 microns 0 7 Pa In...

Page 16: ...z 20 A Power line range 180 to 264 VAC 60 or 50 Hz single phase 30 A To reduce the risk of electrical shock this equipment uses a three wire electrical cord 1 8 m 6 ft and plug to connect the equipment to earthground In regions where the instrument is supplied with an unterminated cord a plug that meets local electrical and safety requirements must be supplied Contact your local Beckman Coulter of...

Page 17: ...end about 5 1 cm 2 in beyond the instru ment The pads under each foot have been designed to prevent possible rotation of the instrument in the event of a rotor mishap A 15 2 cm 6 in clearance is required at the rear of the instrument for servicing the control panel and to ensure sufficient air ventilation during operation The instrument will operate within specifications in a laboratory with ambient...

Page 18: ... Finish urethane paint on top surface general purpose paint on other surfaces coated polycarbonate finish on control panel Maximum heat dissipation into the room 1 0 kW 3400 Btu hr Humidity restrictions 95 non condensing Noise level measured 0 91 m 3 ft in front of the instrument 57 dBa Installation category II Pollution degree 2 cm in 94 0 37 5 1 2 5 1 2 67 3 26 5 15 2 6 ...

Page 19: ...he neck of the control head for your convenience see Figure 1 1 Pressing the POWER OFF button trips the circuit breaker to the off position To return power to the instrument the circuit breaker must be returned to the up position STANDBY MODE When the instrument is at rest it automatically goes into standby mode In this condition only minimal power is used by the instru ment To restore the instrum...

Page 20: ...keypad The keypad is used to enter numerical values It consists of the numbers 0 through 9 a _ a clear entry key and an key DISPLAYS Digital displays indicate rotor speed run time rotor temperature the ω2 t value when in that mode and the selected program number if used The displays serve a dual purpose VACUUM µ SPEED RPM TIME HR MIN TEMP C POWER OFF X 10 000 Location of Diagnostic Messages Parame...

Page 21: ...rns to showing actual conditions Set values remain in memory until new values are entered Whenever you press the instrument displays the set values for 5 seconds then returns to showing actual conditions RUN STATUS INDICATORS Run indicators are provided in the form of green and red LEDs to indicate the status of the instrument Figure 1 1 shows the location of the LEDs on the control panel and Tabl...

Page 22: ...OP A green LED on the _7834a key lights when either the key is pressed or the rotor begins to decel erate It blinks until the rotor comes to a stop ACCEL MAX or SLOW indicates which acceleration profile has been selected DECEL MAX SLOW or NO BRAKE indicates which deceleration profile has been selected ω2 t ω2 t indicates that the ultracentrifuge is in the ω2 t mode HOLD HOLD indicates that the ult...

Page 23: ...ressing _ 991a before the door can be opened After the door is opened the chamber is brought to approximate room temperature to prevent condensation from collecting in the chamber To help keep the chamber dry and clean the door should be kept closed whenever possible A purge system removes significant amounts of moisture from the vacuum pump and chamber If it takes a long time to pull a vacuum howe...

Page 24: ...um system is turned on The rotor temperature is controlled to 1 C of the set value If the temperature system malfunctions the TEMP diagnostic message will appear and the rotor will decelerate to a stop OVERSPEED SYSTEM The overspeed system is a safety feature designed to ensure that the rotor does not exceed its maximum allowable speed This system includes photoelectric sensors in the rotor chambe...

Page 25: ...d rotor energy is determined to be excessive a SPEED diag nostic message will appear and the instrument will immediately shut down thus providing a redundant safety check DRIVE The instrument uses a frequency controlled air cooled direct drive induction motor that requires no gears or brushes In addition the drive does not require an oil vacuum seal external oil reservoir or continuously operating...

Page 26: ...or modify the value in that field While a field is still blinking you can retrieve the original value after having modified it by pressing that parameter key again The original value appears but the field continues to blink so that you can either enter a new value or press _ 28 6a to retain the original one Press _ 28 6a or another parameter key to save the entry If the entry is valid the field will st...

Page 27: ...r a diagnostic message Press _ a a second time to exit from the editing mode The field will stop blinking and the actual values will reappear Press to enter a setting into memory Press and _78 68a within 5 seconds to start a run Press during a run to display the settings for 5 seconds After 5 seconds the displays show the actual conditions once again Press and _7834a within 5 seconds to display the...

Page 28: ... set speed is checked against the overspeed disk If the speed setting is greater than that allowed by the overspeed disk the run will continue but the rotor speed will be lowered to the maximum speed permitted by the disk The TIME setting is also changed to provide an equivalent ω2 t value if in the ω2 t mode A SPEED diagnostic message will appear indicating that these changes have been made Press...

Page 29: ...and _78 68a to begin the run the instrument checks to make sure that a time entry has been made The instrument cannot proceed without a valid entry for TIME ω2 t or HOLD If you have not made an entry the TIME field blinks and you must enter one before the run can proceed The time begins counting down when the rotor begins to accelerate When the time remaining reaches zero the rotor automatically de...

Page 30: ...hours and 59 minutes four dashes will appear in the TIME display After the run pressing _7834a and in succession will show the accumulated value for ω2 t in the SPEED display when the rotor began to decelerate After 5 seconds the display returns to showing current conditions The ω2 t LED will remain on until a different run mode is selected as a reminder that the instrument is still in the ω2 t mo...

Page 31: ...nstrument will beep twice Press _ a to clear the entry then enter a valid temperature setting 3 Press another parameter key or to save the temperature setting You may change the set temperature at any time by repeating the steps in the example The rotor temperature will be adjusted accordingly Actual rotor temperature after equilibration is controlled to 1 C of the set value If the temperature con...

Page 32: ... the program number 1 to 9 using the keypad or by pressing the _463 a key repeatedly until the desired set of parameters appears This is useful if you do not remember the contents of a particular program or to scan through available programs Follow the instructions below to create a new program or to modify an existing one To create or modify a program 1 Press _463 a If no program has been previou...

Page 33: ...functions _ _ _ _ 9 9 9 99 9 9 91 1 1 1a a a a The vacuum system is activated automatically when you press and _78 68a to begin a run To evacuate the chamber at another time make sure the chamber door is closed then press _ 991a As the vacuum system begins to evacuate the chamber a series of three LEDs labeled 750 200 and 20 microns blink remain lighted or go off to allow you to monitor the change...

Page 34: ...l the run ends or _7834a is pressed _ _ _ _7 7 7 78 8 8 83 3 3 34 4 4 4a a a a Use the _7834a key to do the following Press _7834a at any time to terminate the run A green LED on the key will blink to indicate that the rotor is decelerating a tone will sound when the rotor comes to a stop and the green LED will turn off Press and _7834a within 5 seconds to display the run conditions that existed w...

Page 35: ... try to change it the instrument will signal an error 3 Press The SAVE message blinks to indicate that you can save the program settings 4 Press _7 a to save program 0 5 To exit from the program mode press _ a twice RUNNING PROGRAM 0 WITH A SECOND PROGRAM To run the delayed start program function do the following 1 Press _463 a then _ a on the keypad to access program 0 A blinking zero appears abo...

Page 36: ...ond program begins automatically NOTE If you change a run setting during program 0 the second program is automatically canceled The instrument will stop at the end of program 0 Example To run programs 0 and 3 Press _463 a _ a _ a and Press and _78 68a ENTER RECALL ENTER RECALL ...

Page 37: ...ARNING Do not use the Optima LE 80K in the vicinity of flammable liquids or vapors and do not run such materials in the instrument Do not lean on the instrument or place items on it while it is operating WARNING Normal operation may involve the use of solu tions and test samples that are pathogenic toxic or radioactive Operator error or tube failure may generate aerosols Do not run toxic pathogenic...

Page 38: ...in the HOLD mode or to stop any run for any reason Runs in the timed and ω2 t modes terminate automat ically and the instrument sounds a tone to indicate the end of the run After the rotor has stopped press _ 991a to vent the chamber Open the chamber door and remove the rotor To keep the rotor chamber clean and dry keep the door closed between runs To repeat a run press and _78 68a PROGRAMMED OPER...

Page 39: ...n and dry keep the door closed between runs DELAYED START PROGRAM FUNCTION 1 With the power on open the chamber door install the rotor and close the chamber door 2 Press _463 a Use the keypad to press _ a and then a second program number Press 3 Press and _78 68a The word delay appears in the SPEED display and the second program number appears in the _463 a display When the elapsed time in program...

Page 40: ...re Refer to the applicable rotor manual for complete instructions on rotor preparation use and care Refer to Section 4 TROUBLESHOOTING AND MAINTENANCE if a diagnostic message flashes on the control panel during the run ...

Page 41: ...ailure occurs during the run the rotor begins to decelerate with the brake off If the rotor is still spinning above 1000 rpm when power is restored the instrument will resume operation and the rotor will return to set speed with maximum acceleration A PWR diagnostic message will appear to alert you that a power outage occurred If the rotor is spinning below 1000 rpm or has stopped when the power i...

Page 42: ...e brake may take hours to come to a complete stop To gain access to the rotor it will be necessary to remove the control head cover and the front panel to disengage the door lock 1 Locate the two metal latches see Figure 4 1 at the lower outside corners on the back of the control head Use your fingers to depress the latches to disengage the cover from the control head With both hands lift it slight...

Page 43: ...el listen carefully for any sounds coming from the drive Then touch the fan housing Figure 4 3 to feel if it is vibrating and listen again Do not proceed if any sound or vibration is emitted from the housing ENTER RECALL 7 8 9 4 5 6 1 2 3 0 CE VACUUM SPEED TIME TEMP START STOP Access to Cover Latch Cover Latch ...

Page 44: ...lve and wait because the rotor is still spinning 5 About 10 seconds after the hissing noise stops turn the screw to the left counterclockwise until it stops 6 Push down on the interlock pin Figure 4 3 with your finger while opening the chamber door with your free hand If the rotor is still spinning close the door and wait Be very careful not to touch any wires or circuitry ENTER RECALL 7 8 9 4 5 6 ...

Page 45: ...s and insert the upper edge of the front panel under it Push back gently to engage the tabs then down to activate the latch 9 Replace the control head by sliding it along the small ridges pro vided making sure to clear the emergency POWER OFF switch in front When the control head cover is correctly positioned push it until the latches snap shut and the cover is aligned with the rest of the instrum...

Page 46: ...ide cautionary information that will not shut down a run in progress Others indicate a user error For example if you left the chamber door open when you pressed _78 68a the DOOR message would appear to let you know it must be closed If the associated display is flashing when a diagnostic message appears a shut down malfunction has occurred The run will come to a stop See Table 4 1 to determine the ...

Page 47: ...BER The rotor chamber is coated with epoxy resin paint and the chamber door O ring is Buna N rubber The chamber can be wiped clean with a cloth dampened with a mild detergent such as Solution 555 The O ring should be cleaned with a tissue or soft cloth every 3 or 4 months Replace the O ring whenever it becomes worn or damaged Lightly coat the O ring with silicone vacuum grease 335148 before reinst...

Page 48: ...um system malfunctioning Check the air inlet at bottom of the front panel for obstructions Call Beckman Coulter Field Service if problem persists DRIVE Abnormal change in drive speed or overheated drive Be sure a rotor is properly installed on the spindle if power has failed wait for 5 minutes for drive to cool check for air inlet obstruction VAC Vacuum not being drawn properly Check door O ring f...

Page 49: ...ese surfaces See Chemical Resistances for more information regarding chemical resistance of instrument and accessory materials While Beckman Coulter has tested these methods and found that they do not damage the instrument no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods ...

Page 50: ...orldwide offices are listed at the back of this manual or see Ultra centrifuge Rotors Tubes Accessories BR 8101 available at www beckmancoulter com for detailed information on ordering parts and supplies For your convenience a partial list is given below Silicone vacuum grease 2 oz 335148 Spinkote lubricant 1 oz 306812 Beckman Solution 555 1 qt 339555 Chamber O ring 801778 Logbook for Ultracentrifu...

Page 51: ...d hereby is repaired or modified by persons other than its own authorized service personnel unless such repair is made by others who meet qualifications similar to those required of Beckman Coulter s service personnel or unless such repair in the sole opinion of Beckman Coulter is minor or unless such modification is merely the installation of a new Beckman Coulter plug in component for such produ...

Page 52: ...ei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 TURKEY Beckman Coulter Ltd E 5 Yanyol Faith Cad 81410 Soganlik Kartal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309 0090 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Business Park London Road High Wycombe Buckinghamshire HP11 1JU England U K Telephone 01494 441181 Fax 01494 447558 e...