BV200 Plasma Viscometer Mk2/3 Customer User
Manual
Issue 9
th
Sep 2020 - Rev 1.8
Page 42 of 53
6.5. Enzyme Cleaning
How to Prepare Enzyme Cleaning Fluid
1.
Mix 3 drops of the enzyme cleaner concentrate with 4 ml of de-ionised water (1:50 ratio)
in a sample tube with an enzyme label. Replace the cap and mix well. Remove the cap
and introduce the tube to the viscometer along with the steriliser tube (see below) to
complete the daily maintenance. Discard any enzyme not used.
The concentrate is supplied in a 30 ml dropper container. We estimate this dropper will
last up to 10 months. The concentrate remains stable until it is mixed with water when it
then has a 14 day use by date. As the enzyme is only mixed when it is needed this
should not be an issue.
To Commence Enzyme Cleaning
2.
An enzyme clean will commence when a tube displaying an
„ENZYME‟ label is read by
the barcode reader on the viscometer. The probe will go into the Enzyme tube and then
extract all of the Enzyme cleaning fluid. The fluid is retained in the Cassette, Upper
Manifold, and Upper Valves for approximately 4 minutes (during this time the Enzyme
digests the blood product proteins).
The conveyor is then moved and the washpot comes forward and the probe lowered.
Enzyme fluid from the upper manifold is transferred to the washpot, drain line and
valve, and lower manifold, and held for 18 minutes to work on the washpot.
This waiting period can be stopped at any time if necessary. However, this is not
recommended. If it is stopped the whole process should be repeated as soon as is
practicable.