BV200 Plasma Viscometer Mk2/3 Customer User
Manual
Issue 9
th
Sep 2020 - Rev 1.8
Page 20 of 53
5.
Draft Standard Operating Procedure (SOP)
5.1. Before operating the viscometer
Empty and rinse the 4 litre waste bottle.
1.
Empty and rinse the condensation bottle.
2.
Ensure the wash container is emptied, rinsed and then refilled daily with fresh wash.
3.
Wash = 2 litre distilled or de-ionised water with 1 barcoded wash additive.
All viscometer covers and panels should be fitted and closed.
4.
The control vac regulator (sample vacuum) should be between -0.4 and -0.45 bar.
5.
Do not adjust the regulator unless followed by a full manual calibration
6.
The vacuum on the waste bottle vac gauge should be a minimum of -0.7 bar.
7.
(70% vacuum).
Make sure the cap on the waste bottle is fully tightened and the air tubes are fully
8.
plugged in.
5.2. To test centrifuged EDTA plasma samples
-
Ensure all samples to be tested are fully centrifuged (note
– controls do not need to
be centrifuged)
-
Allow all samples and calibrators to stabilise their temperature to room
temperature before testing. This will take longer if samples have arrived by post or
from a courier.
-
Do not store PV samples in a fridge. Plasma samples should be stored at room
temperature and should never be stored in a fridge. If plasma is stored in a fridge,
cryoproteins will precipitate out of the fluid, leading to incorrect results.
-
All calibration and patient sample tubes should be inserted so that the bar code
labels are aligned with the marker on the carriage tube holder.
-
All tubes must be fully pressed down in the holder to avoid tube crashes in the test
area.
Insert a charged
CHECK
tube with cap on in the first
green
holder
1.
Place centrifuged sample tubes into the black holders, starting after the first
CHECK
2.
tube.
Insert a charged
CHECK
tube in each
green
tube holder as the viscometer is filled.
3.
Insert a charged
CHECK
tube in the
green
tube holder after the last patient sample.
4.
Note: If a known High-Risk sample/s are tested (run as a batch), follow it with a
viscometer
STERILISER
tube placed before the stop flag.
Insert a „stop flag‟ behind the last CHECK tube.
5.
To start sample analysis, left-click on the viscometer screen but
ton marked „Start‟. Top
6.
left
Samples and CHECK calibrators may be added at any time. Move the stop flag to the
7.
end of the sample line.
The viscometer can be set to automatically retest a CHECK which has not been
8.
validated.
