6
7
Section 6
The Column Adaptor Plate (CAP)
The Column Adaptor Plate (CAP) interfaces with the Aurum vacuum manifold
to convert the manifold from a plate- to a column-processing system (Figure 6).
The CAP has 18 black luer fittings in a 6 x 3 array and comes supplied with
additional caps. Up to 18 Aurum miniprep columns, 18 Aurum midiprep
columns, or 9 Aurum maxiprep columns* can be accommodated on the CAP
without the need for connectors or other manifold accessories. The CAP will
also accommodate other columns with luer ends.
When vacuum is applied to the manifold, the CAP should self-seat, forming
an airtight seal without the need to press down on the CAP. However, the
application of gentle downward force may occasionally be required to facilitate
seating.
* If more than 6 maxi columns are used, empty the waste collection tray
before the wash step.
Fig. 4. 96-well purification protocol overview.
Bulletin 2667 US/EG Rev A 01-185 0501
Fig. 5. Column Adaptor Plate with and without Aurum vacuum
manifold.
5a: Blow-up view of luer connection between the Bio-Rad mini and
column adaptor plate, 5b: CAP with individual columns (maxi’s, midi’s & mini’s).
Aurum Vacuum Manifold: Cat. #732-6470
For more information, call Technical Service
at 1-800-4BIORAD (1-800-424-6723). Visit us
on the Web at
www.bio-rad.com/aurum/
Vacuum
manifold
Vacuum
filter flask
Vacuum
regulator
Vacuum
source
Aurum
™
Vacuum
Manifold: 96-Well
Purification
Protocol Overview
For complete protocol, consult instruction manual.
Tubing provided is 4 ft long and must be cut into
appropriate pieces before proceeding.
Vacuum Setup Conditions
Vacuum source to manifold
1.
Use tubing to connect vacuum source to
right side
of vacuum regulator unit.
2.
Use tubing to connect
left side
of vacuum
regulator to filter flask.
Rubber stopper of vacuum filter flask must
contain a hole to accommodate serological
(or comparable) pipet.
Vacuum trap must contain additional sidearm
for connection to regulator.
3.
Use tubing to connect filter flask to nozzle
of manifold top.
Vacuum Purification Conditions
When ready to purify
4.
Place A stage in center of manifold base.
5.
Place waste collection tray on top of A stage.
6.
Place manifold top on base to ensure
complete contact.
7.
Place desired Aurum 96-well plate on manifold
top and proceed with appropriate protocol.
Vacuum Elution Conditions
When ready to elute
8.
Remove A stage and waste collection tray
from manifold.
9.
Place B stage in center of manifold base.
10.
Place clean 96-well collection plate on top
of B stage.
11.
Replace manifold top on base to ensure
complete contact.
12.
Proceed with appropriate elution step as
recommended by protocol.
* The Aurum vacuum manifold and stages should
be rinsed with water after each use.
a.
b.
