Section 9
Troubleshooting Guide
Problem
Possible Cause
Possible Solution
Insufficient negative
Open vacuum regulator
Close vacuum regulator
pressure in vacuum
by turning completely
manifold
clockwise
Manifold top not seated
Ensure uniform contact
properly on base
between manifold top
and base
Use A stage for
waste collection
Purification plate or CAP
Press down gently on
not fully seated
plate or CAP to seal
Unused CAP luer fittings
Close unused luer
not closed completely
fittings completely with
caps
Gasket residue preventing Rinse manifold with
even contact between
distilled water
manifold parts
Lower gasket misaligned
Press gasket back into
groove
Physical damage to
Replace vacuum
manifold or CAP (e.g.
manifold or CAP
chips and cracks)
Purge step in purification
If closure of wells or
protocol
columns (e.g. with
sealing tape restores
negative pressure, no
corrective action
needed.
Excessive noise
Purification plate or CAP
Press down gently on
during vacuuming
not fully seated
plate or CAP to seat
Gasket residue preventing Rinse manifold with
even contact between
distilled water
manifold parts
13
Table 1. Pressure unit conversions
To convert from inches of mercury (
““
Hg) to
Multiply by:
millimeters of mercury or torr (mm Hg, torr)
25.4
millibar (mbar)
33.85
atmospheres (atm)
0.03342
pounds per square inch (psi)
0.4912
kilopascals (kPa)
3.385
Table 2. Vacuum manifolds and their preparative formats
12
Vacuum Purification
Mini
Midi
Maxi
96
Aurum Vacuum Manifold
•
•
•
•
Promega Vac-Man
•
Promega Vac-Man 96
•
QIAGEN QIAvac 96
•
QIAGEN QIAvac 24
•
Millipore vacuum manifold
•
