IN 45 100 010 • AA • 11 / 12
Additional information
Intended use
BabyBio S, BabyBio Q and BabyBio DEAE columns are intended for research and process development only. The
columns shall not be used for the preparation of material for clinical or diagnostic purposes.
Safety
Please, read the associated Material Safety Data Sheets (MSDS) for BabyBio columns, and the safety instructions
for any equipment to be used. Note that the maximum back pressure of BabyBio S, BabyBio Q and BabyBio
DEAE columns is 0.3 MPa (3 bar, 43 psi).
Product information
BabyBio S
BabyBio Q
BabyBio DEAE
Target substance
Proteins, peptides
Protein, peptides,
oligonucleotides
Protein, peptides,
oligonucleotides
Resin
WorkBeads 40S
WorkBeads 40Q
WorkBeads 40 DEAE
Matrix
Rigid, highly cross-linked
agarose
Rigid, highly cross-linked
agarose
Rigid, highly cross-linked
agarose
Average particle size
1
(D
V50
)
45 µm
45 µm
45 µm
Ligand
Sulfonate
(-SO
3
-
)
Quarternary amine
(-N
+
(CH
3
)
3
)
Diethylaminoethyl
(-CH
2
CH
2
N
+
H(CH
2
CH
3
)
2
)
Ion capacity
0.18 - 0.25 mmol Na
+
/ml resin
0.18 - 0.25 mmol Cl
-
/ml resin
0.11 - 0.16 mmol Cl
-
/ml resin
Dynamic binding capacity
130 mg BSA/ml resin
2
50 mg BSA/ml resin
3
40 mg BSA/ml resin
3
Column volume
1 ml
5 ml
1 ml
5 ml
1 ml
5 ml
Column dimension
7 x 28 mm (1 ml)
13 x 38 mm (5 ml)
7 x 28 mm (1 ml)
13 x 38 mm (5 ml)
7 x 28 mm (1 ml)
13 x 38 mm (5 ml)
Recommended flow rate
BabyBio 1 ml
BabyBio 5 ml
1 ml/min (150 cm/h)
5 ml/min (225 cm/h)
1 ml/min (150 cm/h)
5 ml/min (225 cm/h)
1 ml/min (150 cm/h)
5 ml/min (225 cm/h)
Maximum flow rate
BabyBio 1 ml
BabyBio 5 ml
5 ml/min (780 cm/h)
20 ml/min (900 cm/h)
5 ml/min (780 cm/h)
20 ml/min (900 cm/h)
5 ml/min (780 cm/h)
20 ml/min (900 cm/h)
Maximum back pressure
0.3 MPa, 3 bar, 43 psi
0.3 MPa, 3 bar, 43 psi
0.3 MPa, 3 bar, 43 psi
Chemical stability
Compatible with all standard aqueous buffers used for protein purification and 70% ethanol.
Should not be stored at low pH for prolonged time
pH stability
2 - 13
2 - 13
3 - 9 recommended pH
3 - 13
Storage
2 to 25 °C in 20% ethanol
2 to 25 °C in 20% ethanol
2 to 25 °C in 20% ethanol
1. The median particle size of the cumulative volume distribution.
2. Dynamic binding capacity determined at 4-minutes residence time (0.25 ml/min in 1 ml column) in 20 mM Na-citrate, 60 mM NaCl, pH 4.0.
3. Dynamic binding capacity determined at 4-minutes residence time (0.25 ml/min in 1 ml column) in 50 mM Tris-HCl, 50 mM NaCl,pH 8.0.
4. Maximum flow rate for aqueous buffers at 20 °C. Decrease the maximum flow rate if the liquid has a higher viscosity. Higher viscosities can be caused by low temperature
(use half of the maximum flow rate for 20% ethanol).
