Bio-Works BabyBio DEAE Instructions Manual Download | Manualshive

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IN 45 100 010 • AA • 12 / 12

Related Products

Product name

Pack size

1

Article number

Prepacked columns

BabyBio Dsalt 1 ml

1 x 1 ml

45 360 101

BabyBio Dsalt 5 ml

1 x 5 ml

45 360 105

OptioBio 40S 10x100

1 x 7.9 ml

55 420 011

OptioBio 40Q 10x100

1 x 7.9 ml

55 410 011

Bulk resins

WorkBeads 40S

25 ml

40 200 001

WorkBeads 40Q

25 ml

40 100 001

WorkBeads 40 DEAE

25 ml

40 150 001

Accessories

Column plug male 1/16”

10

70 100 010

Column cap female 1/16”

10

70 100 020

1. Other pack sizes can be found in the complete product list on www.bio-works.com

Ordering information

Product name

Pack size

Article number

BabyBio S 1 ml

1 x 1 ml
2 x 1 ml
5 x 1 ml
10 x 1 ml

45 200 101
45 200 102
45 200 103
45 200 104

BabyBio S 5 ml

1 x 5 ml
2 x 5 ml
5 x 5 ml
10 x 5 ml

45 200 105
45 200 106
45 200 107
45 200 108

BabyBio Q 1 ml

1 x 1 ml
2 x 1 ml
5 x 1 ml
10 x 1 ml

45 100 101
45 100 102
45 100 103
45 100 104

BabyBio Q 5 ml

1 x 5 ml
2 x 5 ml
5 x 5 ml
10 x 5 ml

45 100 105
45 100 106
45 100 107
45 100 108

BabyBio DEAE 1 ml

1 x 1 ml
2 x 1 ml
5 x 1 ml
10 x 1 ml

45 150 101
45 150 102
45 150 103
45 150 104

BabyBio DEAE 5 ml

1 x 5 ml
2 x 5 ml
5 x 5 ml
10 x 5 ml

45 150 105
45 150 106
45 150 107
45 150 108

Orders: sales@bio-works.com or contact your local distributor.
For more information about local distributors and products please visit www.bio-works.com or contact us at
info@bio-works.com

Bio-Works
Virdings allé 18
754 50 Uppsala
Sweden

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Summary of Contents for BabyBio DEAE

Page 1: ...r basic proteins i e proteins with a high isoelectric point pI while BabyBio Q and BabyBio DEAE columns are suitable for purification of acidic proteins i e proteins with low pI 1 Choose a suitable pH and buffer for the binding of the target protein One pH unit below pI BabyBio S columns or above pI BabyBio Q and BabyBio DEAE columns is a good starting point 2 Connect the column to the chromatogra...

Page 2: ...therefore interact with an ion exchange resin also at the pI The likelihood of binding to either the cation or the anion exchange resin will increase when moving away from the pI Ion exchange chromatography begins with equilibration of the column to establish the desired pH and charging the resin with suitable counter ions to the charged ligands on the resin e g the negative sulfonate groups can i...

Page 3: ...f a sample that has not been properly clarified may reduce the performance and lifetime of the column The sample should be applied under conditions similar to those of the binding buffer 2 Connect the column Cut off or twist off the end at the outlet of the column see Figure 2 Note It is of high importance to cut off the tip at the very end of the cone preferable using a scalpel Incorrect removal ...

Page 4: ...ate may reduce the yield Applied samples should have a pH that gives the target protein a charge that is opposite the charge of the column resin The pH together with the ionic strength in the sample solution might need adjustment for optimal binding 6 Wash After sample application remove unbound impurities by washing the column with 20 30 CV washing buffer or until desired A280 nm absorbance of th...

Page 5: ...m pressure limit onto the first column If possible the maximum pressure of the chromatography system should be set according to Table 2 Remember always to take the system fluidics contribution to the pressure into account Table 2 Recommended maximum pressure settings for BabyBio columns connected in series Notice that the maximum pressure over each column is always 3 bar No of columns in series Ma...

Page 6: ...fication using BabyBio S BabyBio Q and BabyBio DEAE Other buffers can possible be used Buffer Product Buffer composition Binding buffer BabyBio S 50 mM Na phosphate pH 7 0 Binding buffer BabyBio Q 50 mM Tris HCl pH 7 4 Binding buffer BabyBio DEAE 50 mM Tris HCl pH 7 4 Elution buffer BabyBio S 50 mM Na phosphate 1 M NaCl pH 7 0 Elution buffer BabyBio Q 50 mM Tris HCl 1 M NaCl pH 7 4 Elution buffer ...

Page 7: ... jam and thus reduced diffusion rate into the pores A slightly elevated salt concentration reduces but does not eliminate the interactions with the resin by creating a dynamic adsorption desorption equilibrium that allows further diffusion into the resin thus increasing the binding capacity Tuning the flow rate Flow rate is another factor that can be optimized to improve the binding capacity durin...

Page 8: ...endent on purity and recovery requirements as well as properties of the target protein and the sample Using a gradient elution gives increased purity than step elution but step elution may be necessary to obtain the highest possible concentration of the target protein In order to optimize the salt concentration for step elution an initial gradient test run can be carried out to identify a suitable...

Page 9: ...as ion exchange chromatography This can be carried out quickly and easily using BabyBio Dsalt 1 ml or 5 ml columns see Related products BabyBio Dsalt columns are also a useful alternative to dialysis for larger sample volumes or when samples need to be processed rapidly to avoid degradation Additional purification Ion exchange chromatography is a powerful single protein purification step or combin...

Page 10: ...sin using 1 M NaOH applied by a low reversed flow for 2 hours or overnight is often sufficient CIP of the column can be carried out as followed 1 Wash the column with 5 CV deionized water 2 Apply 3 10 CV of 0 5 1 M NaOH for 15 30 minutes Note The contact time is the important factor treatment with NaOH overnight can be necessary if severely fouled 3 Wash the column with 5 10 CV deionized water or ...

Page 11: ... ml 1 ml 5 ml 1 ml 5 ml Column dimension 7 x 28 mm 1 ml 13 x 38 mm 5 ml 7 x 28 mm 1 ml 13 x 38 mm 5 ml 7 x 28 mm 1 ml 13 x 38 mm 5 ml Recommended flow rate BabyBio 1 ml BabyBio 5 ml 1 ml min 150 cm h 5 ml min 225 cm h 1 ml min 150 cm h 5 ml min 225 cm h 1 ml min 150 cm h 5 ml min 225 cm h Maximum flow rate BabyBio 1 ml BabyBio 5 ml 5 ml min 780 cm h 20 ml min 900 cm h 5 ml min 780 cm h 20 ml min 9...

Page 12: ... ml 1 x 1 ml 2 x 1 ml 5 x 1 ml 10 x 1 ml 45 200 101 45 200 102 45 200 103 45 200 104 BabyBio S 5 ml 1 x 5 ml 2 x 5 ml 5 x 5 ml 10 x 5 ml 45 200 105 45 200 106 45 200 107 45 200 108 BabyBio Q 1 ml 1 x 1 ml 2 x 1 ml 5 x 1 ml 10 x 1 ml 45 100 101 45 100 102 45 100 103 45 100 104 BabyBio Q 5 ml 1 x 5 ml 2 x 5 ml 5 x 5 ml 10 x 5 ml 45 100 105 45 100 106 45 100 107 45 100 108 BabyBio DEAE 1 ml 1 x 1 ml ...

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