IN 45 100 010 • AA • 9 / 12
Extra purification step
Optimization of the overall purification process by tuning the binding, washing and/or elution steps is an option.
However, an additional purification step based on another chromatography technique is recommended (see
Additional purification
).
Desalting and buffer exchange
Buffer exchange or desalting of a sample are useful before analysis and/or after chromatography steps such as
ion exchange chromatography. This can be carried out quickly and easily using BabyBio Dsalt 1 ml or 5 ml
columns (see
Related products
). BabyBio Dsalt columns are also a useful alternative to dialysis for larger sample
volumes or when samples need to be processed rapidly to avoid degradation.
Additional purification
Ion exchange chromatography is a powerful single protein purification step or combined with other
chromatography techniques. The overall process needs to be optimized for each purification step.
To find out more about Bio-Works chromatography resins for additional purification, please visit www.bio-
works.com