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4
English
Issue 02 - 07/2001
OPERATION
Introduction
Your UV/Visible spectrophotometer is a stand alone, simple-to-use instrument with a
high-resolution liquid crystal display (LCD), and a comprehensive range of
spectrophotometry measurements can be undertaken. It fulfils the requirements of
the Pharmacopoeia (Appendix).
It works on the basis of light from the deuterium and tungsten lamps being directed
by a fixed mirror through the monochromator inlet slit. This passes through one of
several (dependent on wavelength selected) filters mounted on a filter quadrant; the
filtered light is then directed onto the holographic grating that produces light of the
selected wavelength. The light then leaves the monochromator via the exit slit, and
mirrors focus and direct the light into the sample compartment. This passes through
your cell, containing the sample of interest, and then a defocusing lens to a solid
state detector unit. The resulting signal is then filtered and displayed.
Your spectrophotometer:
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Measures standard absorbance, concentration and % transmission.
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Has stored parameters for DNA, RNA and oligonucleotide quantification and
purity checking, cDNA fluorophore label check, nucleic acid wavelength scan
and a calculation facility for Tm; there is also useful information concerning
nucleic acids in general.
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Has stored parameters for Bradford, Lowry, Biuret, BCA and UV methods for
protein determination; there is also useful information concerning proteins and
amino acids in general.
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Has Application Modes for
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Wavescan (Wavelength Scanning )
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Enzyme Kinetics
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Multiple Wavelength (Equation Entry)
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Standard Curve
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Substrate Concentration
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Can store up to 50 user-defined methods
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Can download results directly via the serial interface for manipulation in Excel
and for data storage and archiving
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Has GLP self diagnostics
A range of accessories further enhances the capability of the instrument.
