Lightwave UV/Vis Diode Array Spectrophotometer
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Note: If running a long kinetics method, it is advisable to leave the unit on for
at least 30 minutes to allow the lamps to fully stabilise, otherwise a slight long
term drift will be apparent.
10.8 Run a Method
The following screen should be displayed:
Pressing REF will zero the instrument and clear the screen.
Pressing TEST will take a measurement (or if kinetics have been selected,
start the run).
Kinetics Run
During the kinetics run the screen will initially show the Start Time less time
elapsed.
Once this reached zero, the first measurement will be taken, followed by more
measurements at each selected interval until the End time is reached.
Each measurement is displayed on the screen in turn. Once all the readings
have been taken the calculated
∆
A/min, correlation coefficient and
concentration will be displayed.
The
∆
A/min is calculated using a Least Squares Fit. You can view the graph
by pressing function key 1, or scroll through the readings using the page up
or page down keys.
To repeat the test, re-press TEST.