15
ISOLATE
RNA Kits
P
la
nt
R
N
A
M
in
i K
it
10,000 x g, 2 min
10,000 x g, 1 min
10,000 x g, 1 min
Add 650µl Wash
Buffer BPR
Place Spin Column
PR2 in Elution Tube
Add 30-80µl RNase-
free water. Incubate
1 min.
Isolated plant RNA
Total RNA isolation from plant tissue
Transfer supernatant to
Spin Column PR1
Homogenize and lyse tissue
with 450µl Lysis Buffer APR
or BPR. Transfer to 1.5ml tube.
max. speed, 1 min
10,000 x g, 2 min
10,000 x g, 2 min
10,000 x g, 2 min
Add 500µl Wash Buffer
APR
Discard pellet
Discard filtrate
Discard filtrate
Discard filtrate
Discard Spin Column PR1
SAVE FILTRATE
Add 1 vol. 70% ethanol
to filtrate. Transfer to
Spin Column PR2 in new
Collection Tube.
