ISOLATE
RNA Kits
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2. Description
ISOLATE RNA Mini Kit is specially designed for the fast and efficient isolation of
extremely pure total RNA from a variety of samples. The kit is compatible with cultured
cells, tissues and bacterial cells. The isolated RNA is ready for downstream applications
such as reverse transcription, real-time PCR and RNA protection assays.
The protocol is easy to follow on a step-by-step basis. The cells are lysed with an
optimized Lysis Buffer, which simultaneously inactivates RNases. The lysate is then
applied to a Spin Column to selectively remove genomic DNA. There is no need to
perform a separate DNase digestion step. The lysis buffer is optimized to efficiently
lyse the cells in a chaotropic lysis buffer. The lysis buffer also inactivates RNases, thus
protecting the released RNA. The RNA is then bound to a silica membrane. Subsequent
wash steps remove the remaining cell debris. Pure RNA is eluted in the final step with
RNase-free water.
3. storage
The ISOLATE RNA Mini Kit should be stored dry at room temperature. Under these
conditions, the kit is stable for 12 months.
4. safety information
Always wear gloves and a suitable lab coat when handling the reagents of this kit. For
detailed information, refer to the material data safety sheets (MSDSs) available on our
website at www.bioline.com.
5. proDuct specifications
starting material
•
Animal tissue (up to 20mg)
•
Eukaryotic cells (up to 5 x 10
6
cells)
•
Bacterial cells (up to 1 x 10
9
cells)
features
•
High purity RNA
•
Rapid protocol: 15-20 minutes
•
Clear, easy to follow instructions
time required
15-20 minutes
Binding capacity
Approximately 100µg RNA
applications
Isolation of RNA from:
•
Animal tissue
•
Eukaryotic cells
•
Bacterial cells
