Product Manual
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6
Lyse cells with 450µl Lysis
Buffer R
eukaryotic cells/Bacterial cells.
Transfer supernatant to
Spin Column R1
Homogenize and lyse tissue
with 450µl Lysis Buffer R.
Transfer to 1.5ml tube.
Grind
Incubate for 3 min at RT
max. speed, 1 min
10,000 x g, 2 min
10,000 x g, 2 min
10,000 x g, 1 min
10,000 x g, 1 min
10,000 x g, 2 min
6000 x g, 1 min
Add 700µl Wash Buffer BR
Discard filtrate
Discard filtrate
Discard filtrate
Place Spin Column R2 in
Elution Tube Add 30-80µl
RNase-free water. Incubate
1 min.
Add 500µl Wash Buffer AR
Isolated RNA
Discard pellet
Discard filtrate
Discard Spin Column R1
SAVE FILTRATE
animal tissue
Add 1 vol. 70% ethanol
to filtrate. Transfer to Spin
Column R2 in new Collection
Tube.
Total RNA isolation