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email:  sales@cairn-research.co.uk   tech@cairn-research.co.uk 

+44(0)1795 590140   www.cairn-research.co.uk

  

9)  One channel is slightly out of focus (chromatic aberration)

Observations & likely cause

•  One channel is slightly out of focus compared to the other.
•  Chromatic aberration results when the focal length changes depending upon the wavelength and 

is predominantly introduced by the objective lens. When imaging two wavelengths simultaneously, 
the phenomenon is 

observed

 using the OptoSplit II, rather than being caused by it.

Resolution

•  The OptoSplit can be supplied with a Corrector Lens kit as an additional accessory.
•  Three weak crown glass lenses are typically supplied, labelled as follows:
•  IO137: Focal length 1346mm
•  IO138: Focal length 2019mm
•  IO139: Focal length 4034mm
•  The corrector lenses are mounted in the auxiliary drop-in holders provided with each kit.
•  Finding the correct lens is a process of trial and error. We recommend starting with the weakest 

lens (IO139) in the out of focus channel.

•  For further assistance on chromatic correction or multi-depth imaging, please contact us.

For all technical support, please contact our support team 

tech@cairn-research.co.uk or call us on 01795 590 140

Summary of Contents for OptoSplit II

Page 1: ...annel images rather than just the overlay Calibrate the unit as per the set up guide and approach troubleshooting firstly with the calibration cube supplied consisting of a 50 50 mirror Make a detaile...

Page 2: ...rs darker on one side known as vignetting Often observed when changing from split mode to single channel bypass mode due to sub optimal mirror positioning Resolution Locate the Trim adjustment on the...

Page 3: ...cause Illumination is inconsistent over both images Often symptomatic of a poorly aligned light source which has not been setup for K hler illumi nation unrelated to the image splitter Resolution Remo...

Page 4: ...ty can be affected Split control should always be turned ANTI CLOCKWISE i e this moves the two images apart for the best split results Resolution Identify which is the reflected shorter wavelength ima...

Page 5: ...email sales cairn research co uk tech cairn research co uk 44 0 1795 590140 www cairn research co uk Flowchart for confirming camera orientation is correct and images are split correctly...

Page 6: ...research co uk 4 Edges of the OptoSplit aperture appear rough Observations likely cause Rough non uniform feature in focus on diaphragm aperture blade Most likely cause is dirt highly magnified on the...

Page 7: ...instructions below This adjustment is required if when initially installed the aperture blades are slightly out of focus due to the individual position of the camera sensor However this adjustment is...

Page 8: ...ing your imaging software Focussing the Camera on the Aperture If the Optosplit II aperture is not in sharp focus then adjust the fine focus on the camera as follows Set an aperture size of less than...

Page 9: ...dentify this error is if the V control moves an image horizontally which indicates the camera is in the incorrect orientation 6 Two emission channels are non parallel Resolution Refer to the OptoSplit...

Page 10: ...al These LS units include longer focal length custom lenses to minimise image distortion and can be used with any camera sensor size Resolution If you are unsure whether you have an LS OptoSplit Large...

Page 11: ...d by differences in intensity of the emitted fluorophores Resolution The OptoSplit can be supplied with an additional Neutral Density kit to reduce the intensity of the brightest channel Four neutral...

Page 12: ...han being caused by it Resolution The OptoSplit can be supplied with a Corrector Lens kit as an additional accessory Three weak crown glass lenses are typically supplied labelled as follows IO137 Foca...

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