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3.1 

Adjusting and Locking the Aperture

The OptoSplit III is supplied with an adjustable rectangular aperture 

that  allows  the  user  to  determine  the  ROI  both  vertically  and 

horizontally.    Once  the  ROI  has  been  defined,  the  aperture  can  be 

locked in place using the aperture adjusters. (See diagram below.)

In order to adjust the ROI, adjust each of the two levers on the input 

port of the OptoSplit III until the desired area is defined on the camera.  

If  you  wish  to  lock  the  aperture  in  place,  then  tighten  the  levers  by 

rotating them clockwise, and to loosen them rotate anti-clockwise.

3.2 

Adjusting the Position of the Images

For successful image separation it is necessary to first define the field of view, 

and ensure the three optical pathways are overlaid. 
It  is  possible  to  separate  the  images  by  rotating  the  Split  control  in  the 

clockwise sense, however image distortion will be evident when overlaying 

the two halves of the image.
The imaging procedure that follows assumes a Triple Filter Cube is installed 

and the full image is being monitored.
To begin, set a convenient sized aperture using the input aperture levers so 

the edges of the aperture are just visible within the field of view vertically, and 

restrict the field to just under 33% of the horizontal dimension.
It  is  also  important  to  note  that  here  the  reflected  image  (that  with  the 

shortest wavelength), is depicted as being to the right of the screen.  You will 

be able to identify this image easily after seperating all three as it will move 

with adjustments to the V1 control, but not adjustments to the V2 control.

Aperture adjusters

Aperture 

mechanism

Turn the aperture handles

clockwise to lock and unlock

the aperture adjusters

Summary of Contents for OptoSplit III

Page 1: ...www cairn research co uk Free Phone 08453301267 UK only Tel 44 0 1795590140 Fax 44 0 1795594510...

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Page 4: ...rt department who are always willing to help e mail tech cairn research co uk Note Please do not adjust any control before reading this manual The OptoSplit is supplied pre aligned and centred please...

Page 5: ...plit III in the Lightpath Operation of the OptoSplit III Adjusting and Locking the Aperture Adjusting the Position of the Images Focussing the Camera on the Aperture Dual and Single Image Modes of Use...

Page 6: ...hin the camera frame Images can be acquired using any imaging software and processed either manually off line or using an appropriate analysis tool such as the Splitview module in Universal Imaging s...

Page 7: ...ces for three 25mm filters These are held in place by locking rings which can be removed using the tool provided To fit a filter remove the locking ring and place the filter orientated so that the arr...

Page 8: ...ular recess es Then simply replace the holder and reassemble the cube and tighten the screws 2 3 Inserting the Cairn Filter Cube into the OptoSplit III The Cairn filter cube is designed to be easily a...

Page 9: ...Page 4 Remove the access panel using the two handles Mounting bracket Replace the access panel using the two handles Mount the filter cube onto the brackect on the interior of the OptoSplit II...

Page 10: ...lear image can be seen the camera should be switched off and removed from the microscope The OptoSplit III should then be fitted on to the microscope with the diaphragm orientated toward the output of...

Page 11: ...cus The image should line up with the edges of the aperture and should not have any rotation or slant in either direction 3 Operation of the OptoSplit III The OptoSplit III uses a single control for a...

Page 12: ...e overlaid It is possible to separate the images by rotating the Split control in the clockwise sense however image distortion will be evident when overlaying the two halves of the image The imaging p...

Page 13: ...ontrol anti clockwise will seperate the reflected and transmit ted images symmetrically on the horizontal axis whilst the Centre image will not deviate Note To prevent calibration of the OptoSplit III...

Page 14: ...ong the vertical axis and allow for adjustments to be made independently of the Reflected image Adjustments to the H3 controller will move the Transmitted image along the horizontal axis Adjustments t...

Page 15: ...ken the two hex screws that retain the output rotating ring to allow the camera to turn in the C mount 3 Slightly loosen the single hex screw which locks the focussing ring 4 Rotate the focussing ring...

Page 16: ...erture Single image or bypass mode is the simplest of these only requiring the field diaphragm to be fully opened to enable operation in full frame mode When using the OptoSplit III with the regular O...

Page 17: ...e size to optimise the sensor use then readjustment of the split control will allow the outer edges of the images to be repositioned 3 5 Locating the OptoSplit images on the camera If the OptoSplit II...

Page 18: ...ra field horizontally Locating the Transmitted Image 1 Move the shutter from the transmitted to the reflected illumination pathway the calibration cube should remain in place 2 With the reflected imag...

Page 19: ...ontrol 4 If after scanning the horizontal axis no image can be found again open the input aperture slightly repeat step 3 5 Once the edges of the aperture have been found reduce the input aperture as...

Page 20: ...he slider is moved too far then the vignetting will become apparent on the other image and will need to be adjusted in the opposite direction until both images are the same intensity It is important t...

Page 21: ...e to give precise symmetrical control of the degree of separation whilst maintaining identical path lengths A half size fully silvered mirror at the output port for recombining the images Fine adjustm...

Page 22: ...age 17 5 Technical Support E mail tech cairn research co uk Web http www cairn research co uk Address Cairn Research Graveney Road Faversham Kent ME13 8UP Telephone 44 0 1795590140 Fax 44 0 1795594510...

Page 23: ...Page 18 In addition to designing and manufacturing our own products we are also proud distributors for the following companies...

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