CAMAG DBS-MS 500, Instruction Manual

Page 1: ...INSTRUCTION MANUAL DBS MS 500...

Page 2: ...15 2 5 Priming 16 3 Getting started 18 3 1 The HMI Human Machine interface 18 3 2 Setting up an extraction with HMI support 19 General method settings 20 Sample settings 20 Image processing settings 2...

Page 3: ...Change of X Y drive battery 33 Change of extraction head 33 Fittings and capillaries 34 Installing high pressure fittings 34 Fittings dead volumes 34 Fittings System leaks 35 Exchange of capillaries...

Page 4: ...cards are stored back in the rack position they were picked from With the image processing system multiple quality factors and identification features of each card will be measured and checked to ensu...

Page 5: ...much easier from a logistic point of view than adding the internal standard before the blood sample is spotted onto the DBS card CAMAG has been manufacturing high precision application instruments for...

Page 6: ...DBS MS500 loop connection 3 and 8 of loop valve Meanwhile the flow of the LC pump is bypassed through connection 4 and 7 Fig 2 Fluidic principle diagram in position Elution Fig 3 shows the fluidic pri...

Page 7: ...Fluidic principle diagram in position Inline 1 4 Precautions Please read this operating manual before starting the installation This manual contains information and warnings the user has to follow to...

Page 8: ...Do not use extension cords without ground contact When working with the fluids of the instrument be sure to take the appropriate caution protect your eyes from direct contact with liquid Risk of fing...

Page 9: ...the transport security devices if applicable transport it only in the original packaging The safety of any system incorporate with the equipment is the responsibility of the assembler of the system W...

Page 10: ...ssed air 672 0029 1m Tubing PVC to waste canister 672 1016 2 Tubing clamp 663 1002 1 Seal 720 3095 1 Tubing ferrule 735 3008 2 Vat for solvent bottles 960 0062 2 Bottle for extraction rinsing 500ml 96...

Page 11: ...d 450 mm in front to open the door Height 660mm plus solvent bottles and capillaries Plus adequate space for the waste canister underneath the instrument Place the instrument at a solid desk with at l...

Page 12: ...place the instrument near gas burners electric heaters or ovens Do not place the instrument near equipment that generates intense magnetic fields such as electric welding equipment high frequency furn...

Page 13: ...Unpacking Installation Instruction Manual Sep 18 12 5 Move the plate of the shipping protection to the front and 6 Mont the spring of the gripper arm...

Page 14: ...tion solvent Fill and connect the solvent bottle 2 500ml tube Elution 2 and E1 R3 with your appropriate elution solvent Fill and connect the rinsing liquid bottle 1 1000ml tube Rinsing R1 with your ap...

Page 15: ...Unpacking Installation Instruction Manual Sep 18 14 Fig 5 Fluid Sel F V1 Loop F V2 CDM F V3 valvs Port 4 From LC Pump Port 7 To LC MS system Fig 6 Loop valve F V2 connection scheme...

Page 16: ...ake sure you keep the length of the hose as short and the diameter as big as possible Connect the required contact closure according table 1 green additional information about the contact closure you...

Page 17: ...he original delivered CAMAG power cord Connect the LAN cable to the computer for use of CHRONOS software Switch on the unit 2 5 Priming Prior to first use or after a longer period of non using the ins...

Page 18: ...Needle Activate the priming Repeat the steps above until no more air bubbles are visible in the IS syringe Priming of IS cleaning circuit Choose manual Choose Internal Standard Set the time parameter...

Page 19: ...ons 6 Extractor EX Rinsing Priming Internal Standard IS Priming Cleaning Park Test Function Service Functions 7 Password I O Signals 7 1 Adjust Kinematic 7 2 Adjust Camera Import Parameter Parameters...

Page 20: ...e down front side with DBS spots facing towards the back of the instrument to extract into the racks in a continuous way Fig 9 DBS card front side upside up Switch the instrument on Wait until the boo...

Page 21: ...struction Manual Sep 18 20 General method settings Choose Method Editor Fig 11 Method editor screen Enter a description Editor Name and Company if applicable Sample settings Choose Sample List Fig 12...

Page 22: ...he instrument No of samples per card Informs about the maximum number of extractions at one card up to 4 extractions Examples If 1 is chosen and 4 spots are active shown black on the sample list scree...

Page 23: ...tings LC MS Cycletime Expected shortest cycle time when no information from the measurement system is received to start new cycle Spot Type Blood Plasma Blank Card Type Manufacturer cards Custom Card...

Page 24: ...DBS cards this is 13 7mm Offset X Offset value for left right direction Offset Y Offset value for top bottom direction With dashed ring Set it active when card has dashed rings Dashed ring diameter S...

Page 25: ...extract anyway If active the instrument extracts the spot although the spot is not within the below specified specification limits Checkbox Test Checkboxes are taken into account if active Extraction...

Page 26: ...tandard Fig 17 Internal Standard screen Settings IS Bottle No Choose Is bottle for IS application IS Dry Time Time between spraying IS and extraction Cleaning Time Time after each IS application to wa...

Page 27: ...icable Choose properties next to Extraction Fig 18 Extraction screen Settings Elution Process Flow Rate Flow rate for the extraction process Volume Volume to be eluted Bottle No E Choose the appropria...

Page 28: ...Inlet is chosen Rn Choose the appropriate rinsing solvent 1 5 2 rinsing 2 extraction and one rinsing spare solvent Active when Outlet is chosen Rinsing Time Rinsing time Accept parameters with ok Adv...

Page 29: ...to perform them in one run Therefore you have to set up the methods and name them respectively Fig 20 Method Stack screen Choose Stack Editor Choose Add Method Click on the method to be added Choose O...

Page 30: ...samplers Several data system interfaces allow integration into existing systems Chronos for CAMAG exchanges data with other systems and controls all of the processes On the basis of intelligent time m...

Page 31: ...ning would be to follow the decontamination instruction outlined below 4 2 Decontamination Before transportation or a longer term of not using your system decontaminate it in an appropriate manner The...

Page 32: ...n 2 Switch off the DBS MS 500 Remove the extraction head and sonicate it during 10 minutes in methanol water 70 30 3 Take a lint free wipe dip it in the methanol water 70 30 cleaning solution and manu...

Page 33: ...acuum pump are normal and have to be wiped off as well Cleaning the catch basin Switch off the instrument and remove the power cord Remove both back panels of the instrument Remove the electrical conn...

Page 34: ...ntenance is mandatory to keep the instrument in perfect working condition CAMAG recommends executing regular maintenance by a qualified service person for in detailed information about service and rep...

Page 35: ...ing tube Insert the assembly into the receiving port pushing the tubing into the port until it bottoms out Using the delivered wrench tighten an additional to 1 turn past finger tight Remove the fitti...

Page 36: ...e part of the system that can cause problems Check to make sure your tubing is seated properly When using finger tight fittings the tubing must bottom out in the receiving port before the nut and ferr...

Page 37: ...f all the nuts and ferrules you are using so that you can replace them quickly and avoid un necessary down time Check the receiving port for damage Sometimes a leaking connection has nothing at all to...

Page 38: ...on IP IP20 In output connections 2 x RJ45 Power supply Gas supply Service connector CAMAG Switzerland Sonnenmattstrasse 11 CH 4132 Muttenz 1 Telephone 41 61 467 34 34 Fax 41 61 461 07 02 E Mail info c...

Page 39: ...filter rinsing extraction bottles 1 year 666 0029 Solvent filter solvent rinsing bottles 1 year 115 5138 Extraction head Upon need 115 5137 Ambos Upon need 685 5040 CDM valve F V3 Upon need 1 000 000...

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