Application hints
Instruction Manual, Feb-17
17
port, the tubing pocket beyond the ferrule will be completely filled to help avoid dead
volume in the connection.
Tubing sleeves also offer several side benefits. One is the structural support sleeves provide
to the outside of the capillary tubing, helping prevent damage to the tubing as it leaves
the fitting. Furthermore, the extrusion process generally allows a more concentric
connection than precision drilling and machining can provide, allowing for more accurate
tubing-to-thru-hole alignment and decreasing inline turbulence and mixing. For all these
reasons, using a tubing sleeve to connect capillary tubing into a receiving port is the
method of preference - unless the receiving port is specifically designed for use with the
size of tubing you are using!
6.3
Dead volumes
One of the concerns you will have as a chromatographer is about the dead volume
existing in your system. Dead volume is described as a small space within your HPLC
system where remixing of the sample may occur or where your sample can be diluted with
mobile phase. Dead volume can cause critical problems in your analysis of chemical
compounds, as it may bring about band broadening or split peaks in the end result,
making it difficult to obtain good data on your samples. You must keep this in mind when
making connections throughout your system.
Fig. 10: Potential dead volumes in capillary-based applications
The tube diameter is too small
Mixing chamber
The tube is
too short
Summary of Contents for TLC-MS INTERFACE 2
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