Application hints
Instruction Manual, Feb-17
23
Fig. 16: Same plate as in Fig. 15, partially extracted with CAMAG TLC-MS Interface 2.
Resulting detection limits
Detection limits in MS strongly depend on the ability of substances to be ionized, as well
as on the selected ionization source (mainly ESI, APCI or APPI).
If unknown substances have to be identified, the MS has to be run in “scan” mode, and
the TIC (total ion current) is registered.
Known substances can be measured with a much higher sensitivity, if only the ion of
interest (e.g. the ion with a m/z of 195.1 Da in the case of caffeine). In this case, the
extracted substance can be measured in the SIM mode (single ion monitoring), or in the
SRM mode (single reaction monitoring) in the case of MS/MS experiments.
Identification of substance (caffeine) by means of MS
Fig. 17:
Upper part
: Elution of 500 ng caffeine per spot, TIC has been
registered between 80 and 1800 Da.
Lower part
: MS of caffeine with APCI ionization; very low background
noise.
Summary of Contents for TLC-MS INTERFACE 2
Page 1: ...INSTRUCTION MANUAL TLC MS INTERFACE 2...
Page 27: ......
