Application hints
Instruction Manual, Feb-17
19
6.5
Application example
Sample: diclofenac in methanol at a concentration of 0.113 mg/mL
Layer: 20x10 cm HPTLC plate Si60 F254, prewashed with methanol; UV 254 nm
Application: 1, 2, 4, and 10
μ
L as 8 mm bands
Mobile phase: toluene – ethyl acetate – acetic acid 50 : 40 : 1 (v/v/v)
Development: ADC2, saturated chamber, 70 mm from lower edge of plate
Under UV 254nm mark zones with a soft pencil.
Fig. 11: Different volumes of 0.113 mg/mL diclofenac in methanol ; 8 mm bands
MS measurement
Start the HPLC pump.
The eluent used in the presented example was 95% methanol – 5% water and 0.02%
formic acid, with a flow rate of 0.2 ml/min.
Start the MS for data acquisition only (no injection).
The following MS settings were used:
Source
APCI
Mode
positive
Capillary Voltage
4'000 V
Charging V
2'000 V
Corona Voltage
4'000 nA
Drying Gas Temp.
350°C
Vaporizer Temp
250°C
Nebulizer
5 l/min
Nebulizer Pressure
20 psig
Fragmentor
100 V
Mass Range
100 – 500 m/z
Scan
20 min
Summary of Contents for TLC-MS INTERFACE 2
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