Application hints
Instruction Manual, Feb-17
20
Place the elution head over the first sample zone, lower the elution head and switch the
valve to elution position. Make a note of the time, when the elution started. After approx.
1 to 1.5 minutes, the elution is finished. Switch the valve to rear position to stop the
elution. Lift the elution head and press the cleaning button 2-3 times. Place the elution
head over the next sample zone and repeat the process.
This procedure allows you to register a series of samples into one MS file.
Fig. 12: Elution of three consecutive sample zones of diclofenac; 2, 4 and 10
L per 8
mm band. Upper curve TIC, lower curve SIM.
Alternatively you can start the MS for each individual elution, which provides an individual
MS file for each sample.
Fig. 13: Spectrum of a diclofenac zone with 10 µL per 8 mm band
Separation of Caffeine, Paracetamol, Acetylsalicylic Acid
Sample solutions
A stock solution containing 3 mg/ml caffeine, paracetamol, acetylsalicylic acid in ethyl
acetate was prepared and diluted to
1
1'500
µg/ml
2
750
µg/ml
3
300
µg/ml
4
100
µg/ml
5
50
µg/ml
Summary of Contents for TLC-MS INTERFACE 2
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