Getting started
Instruction Manual, Feb-17
7
3.3
Functional principle
The instrument extracts zones (round or oval) from TLC/HPTLC plates or aluminium foils
up to 20 x 20 cm. Plates can be positioned accurately and analysed zone by zone. Elutions
are possible with appropriate solvents, using the standard flow speed of the HPLC-MS
system (e.g. 0.1 ml/min). The system allows a semi-automatic operation involving piston
up/down movement, cleaning and backflushing of the elution head, manual positioning
and valve switching.
The elution head has two connections on the top side, one inlet and one outlet. On the
bottom surface there is a cutting edge seal with a height of about the thickness of the
plate layer. When the elution head is pressed onto a foil or glass plate the cutting edge
seal cuts into the adsorption layer and creates a leak-free seal.
In bypass position solvent flows directly to the MS-System. With the help of a laser
crosshairs the extractor head is easily positioned on a selected zone.
If the position of a zone is not visible, positioning is done with the help of rulers on the
instrument table.
Fig. 3: TLC-MS Interface 2 in bypass position
After lowering the piston, switch the valve to elution position. The solvent passes through
the elution head onto the silica gel, elutes the sample and transports it directly to the
LC/MS-System.
TLC/HPTLC plate or foil with separated substance zone
Force controlled
piston
in upper position
HPLC Pump
delivering
solvent
Laser projects a
crosshairs
Bypass
Frit
Summary of Contents for TLC-MS INTERFACE 2
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