Cellink 000000020356, User Manual

Page 1: ...BIO X 10 mL Pneumatic Printhead User Manual ...

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Page 3: ...d warnings 10 4 Getting started 11 4 1 Unpacking and installation 12 4 2 Raising and lowering the printhead 13 4 3 Loading the bioink cartridge 14 4 4 Your first bioprint 16 4 5 Removing the printhead 23 4 6 Printing optimization 25 5 Relevant G code commands 27 6 Frequently asked questions 31 7 Maintenance 35 Support information 37 ...

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Page 5: ...1 Package contents 01 ...

Page 6: ...2 01 Package contents Item Part number Quantity BIO X 10 mL Pneumatic Printhead 000000020356 1 Air adapter connector 10 mL 000000610041 1 ...

Page 7: ...3 Technical specifications 02 ...

Page 8: ...Material composition External surfaces Powder coated aluminum Internal surfaces Aluminum Recommended operating temperature 20 23 degrees Celsius NOTE We recommend operating the BIO X in temperatures between 20 and 23 degrees In normal operating conditions the chamber temperature of the BIO X will rise 2 3 degrees above room temperature and the 10 mL Pneumatic Printhead temperature will rise above ...

Page 9: ...ded conditions 26 32 degrees Heating capacity and rate Total heating range 30 65 degrees Heating rate Room temperature 40 degrees 10 minutes Heating rate 40 65 degrees 12 minutes Cooling capacity N A Build volume X Y Z 128 x 85 x 83 4 A mm A represents tip length ...

Page 10: ...ssumes a nozzle length of zero Exact build volume depends on cartridge position in printhead and nozzle needle used Build volume may be lower when combining the Pneumatic Printhead with a printhead that restricts printbox movement Please consult the printhead manual for the printheads being used for specific limitations All measurements are in mm ...

Page 11: ...ightness of tip X Y and Z are measured from the front left corner of the printbed Tip Type Tip Length Nozzle Needle Length X Y Z Build Volume Conical 3 2 cm 3 2 cm 12 7 cm 9 36 cm 5 14 cm 611 cm Blunt 2 4 cm 0 635 cm 12 7 cm 9 36 cm 5 94 cm 706 cm3 Blunt 3 0 cm 1 27 cm 12 7 cm 9 36 cm 5 34 cm 635 cm3 Blunt 4 3 cm 2 54 cm 12 7 cm 9 36 cm 4 04 cm 480 cm3 Micron S 1 9 cm 1 9 cm 12 7 cm 9 36 cm 6 46 c...

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Page 13: ...9 Safety information 03 ...

Page 14: ... the printhead mount manually will damage the motor If the mount needs to be moved place the printhead in the loading position described in the Getting Started section Use the proper method to load and unload the 10 mL Pneumatic Printhead onto the BIO X printhead mounts Instructions are outlined in the Getting Started section Do not use a jerking motion to remove the printhead Using a jerking moti...

Page 15: ...11 Getting started 04 ...

Page 16: ...Printhead to the BIO X printbox Align the printhead above the desired printhead mount Figure 2A and push downward using one hand Secure the bottom of the prinhead mount with your opposite hand to avoid straining the motor Figure 2B C D A B C D Figure 2 Attaching the Pneumatic Printhead to the BIO X A Line up the printhead with a mount on the printbox B Slide the printhead down with one hand and se...

Page 17: ...te to the Utilities menu and then go to the Tools submenu 2 Lower the desired printhead mount into the active or loading position by pressing the down arrow Figure 3 3 Press the up arrow to return the printhead to the inactive position Figure 3 Lowering the printhead into the active position ...

Page 18: ...igure 2 2 Fill a cartridge with bioink or obtain a prefilled cartridge Remove the bottom cap if using a prefilled cartridge Figure 4A Attach a nozzle Figure 4B and remove the end cap Figure 4C 3 Align the cartridge adapter to the loaded cartridge Figure 4D Twist until secure Figure 4E A B C Figure 4 Preparing a prefilled bioink cartridge for bioprinting D E ...

Page 19: ...inthead in the active position lowered as explained in the Raising and lowering the printhead section page 13 Figure 5A 5 Connect the air tubing to the air inlet on the printbox above the respective printhead Figure 5C 6 Raise the printhead to the inactive position Section 4 2 Figure 5 Attaching the air adapter to the cartridge and attaching to the printbox A B C ...

Page 20: ...arted 4 4 Your first bioprint 1 Prepare the printhead and load the bioink as detailed in Section 4 3 2 Select Bioprint from the Start menu Figure 6 Figure 6 Select Bioprint on the Start menu and proceed to print setup ...

Page 21: ...d 3 Select an STL file filename stl or G code file filename gcode from the Model menu Figure 7 Proceed to the next menu by selecting Surface along the bottom of the screen Figure 7 Selecting an STL file from the Model menu ...

Page 22: ...18 04 Getting started 4 Select a surface to print on e g a Petri dish and proceed to the next menu Figure 8 Figure 8 Select a print surface from the Surface menu ...

Page 23: ...tions that have been set up Ensure that Pneumatic 10 mL is selected under the printhead type Figure 9 6 Enter your printhead parameters Figure 9 and proceed to the Layers menu Parameters needed for the Pneumatic Printhead include Nozzle diameter Pressure Print speed Preflow delay Postflow delay ...

Page 24: ... to the respective layer characteristics and proceed to the next menu Figure 10 Figure 10 Layer menu You can assign toolheads to respective print areas like perimeter infill and support You can select the infill pattern and density and preview the layers ...

Page 25: ...sure setting Figure 11 Press Print to proceed to the calibration page Figure 11 The Print menu is where you can preview your print parameters and test the extrusion rate You can adjust the param eters on this menu Press Print to proceed to the Calibration menu Press Save to save your configuration as a protocol ...

Page 26: ...Figure 12 Bring the tip of the nozzle needle to your desired starting position We recommend making sure that the tip touches the surface of your printing substrate 10 Start the bioprinting process Figure 12 Figure 12 Use the Bioprint menu to calibrate the system and start the bioprinting process ...

Page 27: ... the cartridge from the top 3 Twist the cartridge pneumatic adapter and lift it off the cartridge to disconnect the cartridge from the pressure supply 4 Cap your cartridge with the top and bottom caps and store it 5 To remove the printhead stabilize it using two fingers on the top surface of the printhead mount Figure 13A Press your thumb against the bottom side of the printhead to push the printh...

Page 28: ...24 04 Getting started Figure 13 Removing the Pneumatic Printhead from the BIO X A B ...

Page 29: ...damage or rupture Pneumatic based extrusion systems enable users to control the pressure within the cartridge and the resulting cell viability However pneumatic based extrusion systems offer less control over the flow rate and filament diameter The diameter of a filament extruded by the Pneumatic Printhead depends on nozzle diameter applied pressure temperature and translation speed of the printhe...

Page 30: ...achment to the substrate It might not be ideal to use a layer height equal to the nozzle or needle diameter because the bioink could expand after extrusion or contract as it gels or self assembles This parameter is both bioink and nozzle dependent and must be evaluated individually for each bioink For depositing droplets please follow the instructions on the droplet printing menu To optimize cell ...

Page 31: ...27 Relevant G code commands 05 ...

Page 32: ...ute move command The value for the Z parameter is the coordinate in mm directing where to move E tells the BIO X to open the valve for extrusion The F parameter is the speed of the printhead in mm min G4 Snnn Pnnn The G4 command tells the system to dwell The S command is the wait time in seconds and P is the wait time in milliseconds G7 Xnnn Ynnn Ennn Fnnn When used in combination with the G90 com...

Page 33: ...the BIO X to open the valve for extrusion The F parameter is the speed of the printhead in mm min G92 Xnnn Ynnn Znnn G92 sets the current position of the printhead to the specified X Y and Z coordinates If no values are given the position is assumed to be 0 0 0 this will also change the Z position Tx Switch between printhead x Where 0 1 2 designate printheads 1 2 and 3 M771 Tx Pyy M771 turns on th...

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Page 35: ...31 Frequently asked questions 06 ...

Page 36: ...to indicate that it is initializing Once it is recognized the LED will turn blue Blue light can also indicate a temperature below 35 degrees When the temperature of the printhead is between 35 and 50 degrees the light turns yellow to indicate that the printhead is warm When the printhead is above 50 degrees the light turns red to indicate that the printhead is hot What is the mechanism that enable...

Page 37: ...ament is not attaching to the surface while printing Your layer height may be too high or your print speed may be too fast These factors can prevent the filament from attaching to the surface My needle is dragging through my printed structure while moving Your layer height may be too small or your print speed may be too slow These factors can cause thicker filaments that build a print that is tall...

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Page 39: ...35 Maintenance 07 ...

Page 40: ...36 07 Maintenance Regularly clean the printhead with a damp cloth to remove any dust or debris ...

Page 41: ...37 Appendix A Consumables needles and nozzles ...

Page 42: ...01 Needle Green 18 0 5 NZ6200255001 Needle Pink 20 0 25 NZ6200505001 Needle Pink 20 0 50 NZ5201005001 Needle Pink 20 1 00 NZ6210255001 Needle Purple 21 0 25 NZ6210505001 Needle Purple 21 0 50 NZ5211005001 Needle Purple 21 1 00 NZ6220255001 Needle Blue 22 0 25 NZ6220505001 Needle Blue 22 0 50 NZ5221005001 Needle Blue 22 1 00 ...

Page 43: ... Needle Orange 23 0 50 NZ6231005001 Needle Orange 23 1 00 NZ6250255001 Needle Red 25 0 25 NZ6250505001 Needle Red 25 0 50 NZ5251005001 Needle Red 25 1 00 NZ6270255001 Needle Clear 27 0 25 NZ6270505001 Needle Clear 27 0 50 NZ5271005001 Needle Clear 27 1 00 NZ6300255001 Needle Lavender 30 0 25 NZ6300505001 Needle Lavender 30 0 50 ...

Page 44: ...0 NZ5340255001 Needle Green 34 0 25 NZ5340505001 Needle Green 34 0 50 Table 3 Compatible conical nozzles Part Number Type Color Gauge Length inch NZ4180005001 Conical Green 18 1 25 NZ4200005001 Conical Pink 20 1 25 NZ3220005002 Conical Blue 22 1 25 NZ3250005002 Conical Red 25 1 25 NZ3270005002 Conical White 27 1 25 ...

Page 45: ...on s Conical Light Blue 21 0 75 NZ2230000801 Micron s Conical Purple 23 0 75 NZ2250000801 Micron s Conical White 25 0 75 NZ2270000801 Micron s Conical Red 27 0 75 NZ2300000801 Micron s Conical Black 30 0 75 NZ1150000501 Micron s Conical Blue 150 µm 0 75 NZ1100000501 Micron s Conical Orange 100 µm 0 75 NZ1050000501 Micron s Conical Yellow 50 µm 0 75 ...

Page 46: ...e accessories Part Number Description Quantity CSC011011102 Empty Cartridges 10cc with End and Tip Caps 10 CSC011011502 Empty Cartridges 10cc with End and Tip Caps 30 000000020878 10ml End Caps 30 OH000000010 Female female Luer lock adapter 10 OH000000050 Female female Luer lock adapter 50 ...

Page 47: ...43 Support information Official site Sales Store Support Official site www cellink com Contact support cellink com Contact sales cellink com Web store www cellink com store ...

Page 48: ...www cellink com ...