19
Always make sure that your tubes are dry externally and capped tightly before
inserting them into the Open qPCR. Failure to do so may cause liquids to vaporize and
create deposits on the internal components of the instrument.
3.3 Open qPCR Single Channel System Calibration
The Open qPCR single channel system requires calibration with the Fluorescein Calibrator kit.
The calibration process, including solution preparation, will take approximately 20 minutes to
complete.
Please note that the instrument lid height must not be adjusted at any point during calibration.
If the lid height is adjusted, a re-calibration is required.
PCR tubes used for the calibration process must be the same tubes that will be used for
subsequent test runs.
Fluorescein Calibrator Kit
Table 3.2
Fluorescein Calibrator
# of Calibrations
Quantity
Catalog #
Shipped with instrument
3
1 x 1.3 mL/vial
-
Available for order
6
2 x 1.3 mL/vial
S0110M
Storage Temperature
Store at 4 °C or -20 °C (long term), protected from light. The product is stable for two years
from date of receipt when stored at the recommended temperature.
Materials Required for Calibration
o
Fluorescein calibrator solution
o
Distilled or deionized water
o
200
µ
L pipette
o
200
µ
L pipette filter tips
o
Chai PCR Tube & Cap Strips, 8-Well Strips, Optically Clear, 100
µ
L
o
PCR tube rack
o
Mini centrifuge
