26
5.
Prepare the water strips by aliquoting 25
µ
L/tube of water into 2 new PCR tube strips.
Cap strip tubes tightly and spin down.
Calibration Procedures
Please note that the calibration process must be completed in one sitting.
The heat block and lid temperature rises above 95
°
C. To avoid injury, do not make
direct contact with the heat block, lid, or tubes immediately after a run. Let the surface
cool for a few minutes before removing the tubes. If samples must be removed
immediately, avoid contact with surfaces by extracting tubes with tweezers.
Always wear gloves and protective eye wear when handling reagents or operating the
instrument.
1.
Log into your account through a web browser. On the software home screen, navigate to
Settings
→
Calibration
.
2.
Under Optical Calibration, select
Run Now
.
3.
The calibration time and materials required will be shown on the next screen (Figure
3.12). Select
Begin.
Figure 3.12
4.
The screen will prompt you to prepare 6 new 8-well PCR tube strips (Figure 3.13).
Gather the previously prepared strip solutions and select
Next.
