2 Introducing the NucleoCounter
10
2.1.1 Fluorescence microscope
The compact fluorescence microscope integrated in the NucleoCounter comprises
LED´s (light emitting diodes) as excitation light source, excitation and emission
filters, optics (lenses) and a CCD (charged co upled device) camera. Furthermore,
the NucleoCounter contains advanced software for image processing. The
fluorescence microscope, with a cassette inserted, is shown in Figure 3.
Figure 3.
The NucleoCounter´s compact fluorescence microscope
Before the NucleoCounter fluorescence microscope is activated a NucleoCassette is
loaded with sample and inserted into the insertion slit of the instrument. The lid is
closed in order to exclude external ligh t from interfering with the analysis. T he
measurement is activated, by pressing the “Run” button.
When “Run” is activated, the actuator moves the piston rod down through the
cylinder of the cassette. Hereby, the stained lyzate mixture is transported throug h
the flow system of the cassette. The form and dimensions of the flow channels
facilitate effective mixing of lyzate mixture and stain. Sensors monitor the liquid
flow, and when the stained lyzate mixture has reached the measurement chamber of
the cassette, the actuator stops the piston movement.
The measurement chamber is illuminated by green excitation light from light source.
The green light is passed through an excitation filter before it reaches the
measurement chamber. The excitation filter allows on ly green light of the
appropriate wavelengths for the excitation of PI to pass.
The green light will excite the PI bound to DNA and in return PI emits red
fluorescence light. Some of the green light will pass through the measurement
chamber together with the red fluorescence light. This green light is removed by an
emission filter, which only allows the red light to pass through. Using an optical lens
system the red light is finally focused on the CCD chip of the camera.
Summary of Contents for 900-0300
Page 1: ...NucleoCounter YC 100 User s Guide Revision 1 3 Technology that counts...
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