Cell Counting with NucleoCounter® NC-202™
991-2020 Rev. 1.3 March 2020 6
2.2
The Via2-Cassette™
Figure 1: The Via2-Cassette™ contains two fluorescent dyes, AO (Acridine Orange) and
DAPI (4',6-diamidino-2-phenylindole), deposited in the first part of the flow system
2.2.1
Loading the Via2-Cassette™ cassette
The Via2-Cassette™ is loaded by pressing the white piston down while the cassette tip is
immersed in the cell sample (Figure 3). Pressing the piston creates a vacuum that draws
approximately 60 µl cell sample into the cassette flow system. The Via2-Cassette™ is
designed for microcentrifuge tubes and accepts a minimum sample volume of 100 µl.
Figure 2: Loading the Via2-Cassette™
After loading the Via2-Cassette™, the sample buffer dissolves the fluorescent dyes, AO
(Acridine Orange) and DAPI (4',6-diamidino-2-phenylindole), deposited inside the flow
channels. The dyes instantly dissolve and stain the loaded cell sample.
After loading the Via2-Cassette™, it is inserted into the NucleoCounter® NC-202™
instrument. Pressing the RUN button will execute the cell counting protocol, that directs
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Summary of Contents for NucleoCounter NC-202
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