Cleaver Scientific multiSUB MSCHOICE, Instruction Manual

Page 1: ...MSMINI MSMIDI MSCHOICE MSCHOICEST MSMAXI Instruction Manual Catalogue No MSMINI7 MSMINI10 MSMINIDUO MSMIDI7 MSMIDI10 MSMIDIDUO MSCHOICE7 MSCHOICE10 MSCHOICE15 MSCHOICETRIO MSCHOICEST20 MSCHOICEST25 MS...

Page 2: ...2 Setting up the Horizontal Gel Tanks 9 3 3 Gel Preparation 10 3 4 Gel Pouring 11 3 5 Running the Gel 13 3 6 Gel Staining and Viewing 14 3 7 Solutions 14 Section 4 References 15 Section 5 Trouble Sho...

Page 3: ...uld read the complete manual thoroughly The unit must never be used without the safety lid correctly in position The unit should not be used if there is any sign of damage to the external tank or lid...

Page 4: ...L CAB MSMINIDUO MS7 UV7 MS7 UV10 MS7 UVDAM Pack of 2 2 x MS7 8 1 1mm 8 sample MS7 LG Strips MS7 WP Platform CSL CAB MSMIDI7 MSMIDI10 MSMIDIDUO Units include tank lid and electrodes and include the fol...

Page 5: ...x L MS15 UVDAM Pack of 2 2 x MS15 20 1 1mm 20 sample MS15 LG Strips MS15 WP Platform CSL CAB MSCHOICETRIO MS15 UV7 MS15 UV10 MS15 UV15 MS15 UVDAM Pack of 2 2 x MS15 20 1 1mm 20 sample MS15 LG Strips M...

Page 6: ...x L MS20 UVDAM Pack of 2 2 x MS20 20 1 1mm 20 sample MS20 LG Strips MS20 WP Platform CSL CAB MSMAXIDUO MS20 UV10 MS20 UV20 MS20 UVDAM Pack of 2 2 x MS20 20 1 1mm 20 sample MS20 LG Strips MS20 WP Platf...

Page 7: ...s include dishwashing liquid Hexane and Aliphatic hydrocarbons The units should not be left to in detergents for more than 30 minutes The units should never come into contact with the following cleani...

Page 8: ...ltitude 2 000m Temperature range between 4 C and 65 C Maximum relative humidity 80 for temperatures up to 31OC decreasing linearly to 50 relative humidity at 40OC Not for outdoor Use This apparatus is...

Page 9: ...o that there is no gap between the lid and the leading edge of the cable fitting 4 Refit the lid Instructions for fitting Loading Guides These can be fitted to enhance visibility of the wells if desir...

Page 10: ...multiSUB Maxi Tray 20 x 10 cm 20 x 15 cm 20 x 20 cm Gel volume for a 5mm thick gel 100 mL 150 mL 200 mL 2 Add the agarose powder to a conical flask 3 Add the appropriate amount of 1x TAE or TBE soluti...

Page 11: ...gel leakage 2 Place the comb s in the grooves Each tray has more than one comb grove so that multiple combs can be used Using multiple combs increases sample number available per gel but decreases ru...

Page 12: ...to the edge of the gel and dispersed using a pipette tip 5 Allow the agarose to set ensuring that the gel remains undisturbed 6 Carefully remove the gel casting gates and comb and transfer the gel inc...

Page 13: ...ally 3ul of sample buffer is adequate but less may be used with sample volumes of less than 10ul 2 Fill the unit with buffer until the gel is just flooded with buffer This will give the fastest resolu...

Page 14: ...d water again ensuring the gel is completely immersed 3 Rinse the gel twice for a couple of seconds with distilled water 4 Transfer the gel to a UV Transilluminator 5 The samples will often appear as...

Page 15: ...of 50 glycerol 0 25 bromophenol blue and 0 25 xylene cyanole FF in 1x TAE buffer Only 1 10 ml of the 10x loading dye should be prepared Ethidium Bromide Solution Add 10 mg of Ethidium Bromide to 1 ml...

Page 16: ...the running surface Curved line or distortion of bands Bubbles in sample wells Remove bubbles prior to electrophoresis Curved bands smiles Sample overload Reduce load Differential relative mobilities...

Page 17: ...ng Platform MSMINICP Cool pack and Platform MSMINIBSB Buffer Saver Blocks pk 2 saves 100ml of buffer MS7 UVS 7cm UV Gel Scoop CSL CAB Electrophoresis cable Black Red MSMIDI7 multiSUB Midi 10 x 7cm UV...

Page 18: ...ms MS15 PE Positive Electrode MS15 NE Negative Electrode MSMS15 LG Adhesive Loading Guides MS15 WP Viewing Platform MSCHOICECP Cool Pack and Platform MS15 20 FC multiSUB Choice Maxi Flexi caster MSMAX...

Page 19: ...7 16 0 75 16 MS10 16 0 75 16 MS15 16 0 75 25 MS20 25 0 75 20 MS10 20MC 0 75 18 MS15 18MC 0 75 30 MS20 30 0 75 20 MS15 20 0 75 36 MS20 36 0 75 28 MS15 28MC 0 75 40 MS20 40MC 0 75 30 MS15 30 0 75 multiS...

Page 20: ...5 16 1 5 25 MS20 25 1 5 20 MS10 20MC 1 5 18 MS15 18MC 1 5 30 MS20 30 1 5 20 MS15 20 1 5 36 MS20 36 1 5 28 MS15 28MC 1 5 40 MS20 40MC 1 5 30 MS15 30 1 5 multiSUB Mini 2mm multiSUB Midi 2mm multiSUB Cho...

Page 21: ...supplies as 20 blister packs of 10 x 0 5g tablets CSL RUNSAFE CSL RUNSAFE Package 1 ml vial CSL TBEP Powdered Tris Borate EDTA Running Buffer 10 sachets 1litre pk TBE10X1L Cleaver Buffer Tris Borate...

Page 22: ...dified Units which have accessories or repaired parts not supplied by CSL or its associated distributors have invalidated warranty CSL cannot repair or replace free of charge units where improper solu...