CMI | SOP003_rev1.0
4
6
System Overview
The witec confocal raman microscope consists of the following devices:
6.1
Instrument Control PC, 64 bit running witec control and project software (version 1.6)
6.2
Controller unit.
6.3
Upright microscope with led light source for brightfield work.
6.4
Objectives including a 40x water dipping lens and 100 x air
6.5
Andor CCD cameras
6.6
Witec diffraction gratings for either vis or NIR operation.
6.7
Motorised stage for xyz control
6.8
Fibre optic cable for 633nm use: 50micron diameter
6.9
Fibre optic cable for 785 nm use : 100 micron diameter
Familiarise yourself with the system folders, located on the shelf above the equipment.
7
Start Up Procedure
7.1
Book equipment on CMI web booking system.
7.2
Turn on lasers or individual laser, if only one is to be used. For the 633 laser, turn on the key. For
the 785 nm laser, turn on power first and wait until the green led stops flashing. Then switch the
laser key to ‘on’ and again wait until the green led stops flashing and both yellow and green leds
Fibre to detection
system. Use 50 micron
fibre for 633 nm, 100
micron fibre for 785nm
excitation, fibre
diameter is part of the
serial number taped on
fibre.
Extreme care
needed when handling
flibre cables.!!!
Adjustment
screws for
alignment
Push/pull rod for
eyepiece/detector
Laser coupler
and filter wheel
Eye piece
camera
785nm laser
Brightfield/DIC/Ra
man imaging
selection
