5.0 Operation
5.1 Setting up the illumination
For optimal effect in contrast and resolution one should follow the below procedure:
•
Place a specimen on the object stage and focus using the 4x objective with a fully opened iris diaphragm
•
Turn light intensity to the lowest position, then look through the eyepiece(s) and turn up to comfortable
intensity level
•
Turn the condenser in the highest position (for phase contrast models, please set condenser to brightfield
position)
•
Close the iris diaphragm, until it is just visible on the edge of the field of view
The microscope is properly set for use with the 4x objective. For each other magnification in brightfield use this
procedure should be repeated to ensure the best balance between contrast and resolution. Phase contrast use will be
explained further on in this manual.
Caution:
The maximum light intensity when using the 4x and 10x can damage the eyes!
5.2 Placing the specimen slide
•
Push the arm of the specimen holder backwards
•
Release the arm slowly clamping the slide with the cover glass facing up
•
Rotating the X and Y-axis knob will move the specimen to the center for alignment with the center of the
objective
5.3 Focusing and slide protection
•
Select the objective 4x to the optical path
•
Observe the right eyepiece with right eye. Rotate the coarse focusing knob until the image appears
•
Rotate the fine focusing knob for detailed focusing
•
When focused with S100x objective, lock the slide protection handle. The slide protection handle protects the
slide by limiting the travel of the table. This way the objective will not touch and damage your slides
5.4 Adjusting the focusing tension
The focusing knobs can be adjusted for tension. You can set it
from light to heavy according your own preference. Please note
that when the specimen leaves the focus plane after focusing
or the stage declines by itself, the tension should be set higher.
To tighten the focusing arm (more heavy), rotate the tension
adjustment ring according to the arrowhead pointed; to loosen
it, please turn it in the reverse direction.
Focus tension
Slide protection
10
