6.2 Stage 1 – Rinse storage solution from the cassette
Opening the cassette bag safely
The cassette and cassette bag contain an aqueous solution containing 0.1 to 0.2 N NaOH and 20 to
22% glycerin by weight. When opening the cassette bag, follow the standard safety procedures for
handling aqueous NaOH, including the use of safety glasses, safety gloves, and protective lab coat.
Step
Action
1
To avoid unexpected spilling, hold the cassette package upright over a sink and trim the
top of the cassette bag with scissors. Drain and dispose of the excess storage solution in
accordance with environmental regulations.
2
To avoid inadvertent contact with the storage solution after the cassette is removed
from the bag, rinse the outside surface of the cassette with high purity water.
3
If you allow an ultrafiltration cassette to dry out, the membrane will be damaged.
Therefore, do not store the cassette without rewetting it with an approved storage
solution.
Rinsing the storage solution from the cassette
At a minimum, before using a new or used-and-stored cassette, rinse the storage solution from the
cassette following these steps:
Step
Action
1
Open the cassette bag and remove the cassette following the safety instructions above.
2
Install the cassette in the cassette holder. (See your cassette holder’s user guide for
specific installation instructions.)
3
Configure the system so that the retentate and permeate lines discharge to waste (see
). Close the feed, retentate, and permeate valves.
4
Fill the reservoir with an appropriate volume of clean water (6 liters per 0.11 m
2
[1.2 ft
2
]
cassette).
6 Preparing a Cassette for Use
6.2 Stage 1 – Rinse storage solution from the cassette
28
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI
