cytiva Kvick Lab Cassette Holder, User Manual

Page 1: ...Learning to Use the Kvick Lab Cassette Holder User Manual cytiva com...

Page 2: ...rUse 26 6 1 Introduction 27 6 2 Stage1 Rinsestoragesolution fromthe cassette 28 6 3 Stage2 Measuring waterflux 30 6 4 Stage3 Testtheintegrity of the cassette 34 6 5 Stage4 SanitizingtheKvickLabcassett...

Page 3: ...sions 60 11 2 Materialsofconstruction 61 11 3 Operating parameters 62 12 Appendix 63 12 1 Chemicalcompatibility 64 12 2 Cassettesanitizing Agents 65 12 3 Partsand accessories 66 Index 67 TableofConten...

Page 4: ...d could resultinminorormoderateinjury Itisimportantnotto proceed untilallstatedconditionsaremetandclearlyunderstood Note A note is used to indicate information that is important for trouble free and o...

Page 5: ...d connecttheholdertoyourcross flowsystem howtoinstallcassettes andhowtouse and clean theholderand cassettes In this chapter Section Seepage 2 1 Audience 6 2 2 Where tofind moreinformation 7 2 3 Safety...

Page 6: ...entedinthisguide assumestheuserpossessesbasiclaboratoryandtechnicalskills andhastheknowledgeand documentation tosafely operateanyusersuppliedequipmentconnectedtotheKvickLabcross flow system Ifyouneed...

Page 7: ...use and maintain theKvickLabcross flowsystem reservoir feedpump andaccessories Suppliedwith KvickLabcross flow systems LearningtoUsetheKvickLabCassetteHolder DescribeshowtousetheKvickLabcassette hold...

Page 8: ...tesina mannernotspecifiedbyCytiva youmayimpairtheprotectionprovidedbytheholder and cassettes When using anylaboratory pilot scale orproductioncross flowequipment thepotentialexistsfor personalinjury u...

Page 9: ...ssedairsupplytothemaximumoperatingpressureof yoursystemcomponents FortheKvickLabcassetteholderand KvickLabsystem themaximumoperatingpressureis4barg 60 psig Weartheappropriatepersonalprotectiondevicesw...

Page 10: ...ea secure controlledgripandasufficientnumberofpeople orthe properequipment tomovethecassetteholder Wearsafety shoes Theweightofcassetteholdersrequiresthatyousupport themwell especiallywhenintegratedin...

Page 11: ...eventpluggingtheporesof thecassette alwaysuse0 2 mfilteredorwater for injection WFI whenrinsingorflushingthecassette orwhenmakingupcleaning solutionsoraddingdilutionwatertoprocessfluids Inthisuser gui...

Page 12: ...ion Seepage 3 1 Unpacking and lifting thecassetteholder 13 3 2 Packagecontents 14 3 3 Learning aboutthe holder 15 3 4 Features 16 3 5 Learning aboutcassettes 17 3 KvickLabCassetteHolder 12 LearningtoU...

Page 13: ...tation Tounpackthesystem placetheboxontheground Openthetopoftheboxand removefoaminsert Removethecassetteholder documentation andtorquewrench Whenyou transportthecassetteholder holditfirmlywithtwohands...

Page 14: ...assetteholderincludesthefollowingcomponents KvickLabholder Torquewrench socket and torquewrenchinstructions Userguide Certificateofanalysis 3 KvickLabCassetteHolder 3 2 Packagecontents 14 LearningtoUs...

Page 15: ...andthe cassettesenableyoutoseparate concentrate anddiafiltratebiologicalsolutionswithprecision For example you can completeultrafiltrationatlaboratoryvolumes startingvolumesoflessthan1 liter 3 KvickLa...

Page 16: ...ompetitive economicsofuse Themain componentsincludeaflowdistributionmanifold backplate andtierods seetheFigure below Thesecomponentshold thecassettesinplaceandmanagetheflowofprocessfluidsintoand outof...

Page 17: ...sharedesigncomponents includinginletandoutletports labeling andalignmentnotches seetheFigurebelow Figure 3 2 The main parts of a Cytiva cassette 3 KvickLabCassetteHolder 3 5 Learningaboutcassettes Lea...

Page 18: ...e Holder to Your System In this chapter Section Seepage 4 1 Where toinstallthe holder 19 4 2 How toinstalltheholder 20 4 ConnectingtheHoldertoYourSystem 18 LearningtoUsetheKvickLabCassetteHolderUserMa...

Page 19: ...ion seetheFigurebelow Tolearnaboutalternatesystemconfigurationsforspecial applications contactCytiva Retentate Pump Valve Pressure Feed gauge Kvick Lab holder vessel Collection Permeate Drain line Dia...

Page 20: ...tothe holder Thesystempipingmustendin inchsanitaryclampstoconnecttothe KvickLabholder Three fractionalsanitary clampsand inchgaskets Making the connections PlacetheKvickLabholderon asturdylabbenchnear...

Page 21: ...lder In this chapter Section Seepage 5 1 Introduction 22 5 2 Determining how manycassettesyoucaninstall 23 5 3 Installing thecassettes 24 5 InstallingCassettesintheHolder LearningtoUsetheKvickLabCasse...

Page 22: ...eholderandclampthemin placeusing thetierodspacersandnuts Youmusttightenthenutstoaspecifictorquevalueto ensurethe properclamping forceisappliedtosealthecassetteinplace 5 InstallingCassettesintheHolder...

Page 23: ...etheTablebelow Table5 1 CassetteinstallationguidefortheKvickLabcassetteholder Cassettetype Cassettesize Numberofcassettes Nuttorque KvickLabPacket 100cm2 0 11ft2 1to10 13 5Nm 120in lb KvickLab 0 11m2...

Page 24: ...evenly 5 Tighten eachnutalternately1 4turnatatimewiththesuppliedtorquewrenchand socketuntileach nutistightenedtothevalueslistedinTable 5 1 on page 23above Ifa sealisnotachieved theremaybeacassette gas...

Page 25: ...integral gasket facing the backing plate 5 Tie rod 6 Spacer 7 Washer 8 Nut 9 Guide rod Figure 5 1 Top view of Kvick Lab holder showing assembly 5 InstallingCassettesintheHolder 5 3 Installingthecasse...

Page 26: ...sestoragesolutionfromthecassette 28 6 3 Stage 2 Measuringwaterflux 30 6 4 Stage 3 Testtheintegrityofthecassette 34 6 5 Stage 4 Sanitizing theKvickLabcassette 37 6 6 Stage 5 Conditioning thesystemwithb...

Page 27: ...tandthewettedparts Thebuffercan alsobring the systemtotheproperoperatingtemperaturebeforeprocessingbegins WARNING Thecassetteandcassettebagcontainanaqueoussolution containing 0 1to0 2NNaOHand20to22 gl...

Page 28: ...ater 3 If youallow an ultrafiltrationcassettetodryout themembranewillbedamaged Therefore donotstorethecassettewithoutrewettingitwithanapprovedstorage solution Rinsing the storage solution from the cas...

Page 29: ...ushingthecassette orwhenmakingupcleaning solutionsoraddingdilutionwatertoprocessfluids 6 Open thepermeatevalveandclosetheretentatevalveandpumptheremainingwater through the permeatelinetowaste maintain...

Page 30: ...ction 1 Measurethewaterflowfromthepermeateportattwotransmembranepressures 2 Calculate waterflux inl m2 hr LMH forthetwowaterflowmeasurements 3 Plotthetwoflux values TheninterpolateKvickLabcassettewate...

Page 31: ...permeateflowrateinml min Recordthetemperatureofthewaterinthefeedreservoir 6 Drain thesystem Pump Valve Pressure Retentate Feed Feed gauge reservoir Kvick Lab holder Permeate Drain line cylinder Optio...

Page 32: ...stepstonormalizethewaterfluxvalueto20 Candstandardizeto1psigtoarriveatflux inLMH psigat20 C Step Action 1 MultiplythecalculatedfluxvaluebytheappropriateviscositycorrectionfactorinTable 1 Forexample if...

Page 33: ...ormation Table6 1 Viscositycorrectionfactors Temperaturein C whenpermeate flowwasmeasured Viscosity correctionfactor Temperaturein C whenpermeate flowwasmeasured Viscosity correctionfactor 4 1 567 25...

Page 34: ...our system for leaks Totestyourcross flow systemforleaks fillthesystemwithcleanwater Openthefeedvalve Open theretentatevalve Close the permeatevalve Startthefeedpumponslowspeedandthrottlethe retentate...

Page 35: ...rted graduated with water cylinder filled water filled with Beaker Flexible tubing connected from permeate line and inserted into mouth of inverted graduated cylinder Figure 6 4 Flow diagram for testi...

Page 36: ...g 6 4 on page 35 5 Whentheairbubblerateisstable notethetimeandthestandingairvolumeinthe graduated cylinder When5to10mlofairhascollectedinthegraduatecylinder record thetimeand airvolumeagain 6 Usingthe...

Page 37: ...rate 875ml minforeach0 11m2 1 2ft2 KvickLabcassetteinstalled 4 Circulatethe sanitizingsolutionforminimumof60minutesatatemperatureof35to45 C Stopthepump IfyouaregoingtostoretheKvickLabcassette rinseand...

Page 38: ...lsolutionflowsfromtheretentateand permeatelines 4 Close the retentatevalvetoproduceabout0 3to1barg 5to15psig ofretentate pressure Run untilnobubblesappearinthepermeatestream 5 Open the retentatevalvea...

Page 39: ...ed reservoir Kvick Lab holder Permeate Figure 6 6 Typical configuration for conditioning the system with buffer 6 PreparingaCassetteforUse 6 6 Stage5 Conditioningthesystemwithbuffer LearningtoUsetheKv...

Page 40: ...e 7 1 Introduction 41 7 2 Stage1 Startingthecross flowprocess 43 7 3 Stage2 Operating thesysteminasteadystate 44 7 4 Stage3 Shutting thesystemdownandrecoveringproduct 45 7 UsingtheHolder 40 Learningto...

Page 41: ...page 42 The operating proceduresdescribedinthischapterarebasedonFig 7 1 on page 42 Recommended operating conditions When using yourKvickLabcassette fullyopenthepermeatevalveandadjustyourpumpspeedand r...

Page 42: ...ion vessel Permeate Drain line Diafiltration solution optional Pressure Feed reservoir gauge Figure 7 1 Typical process flow path for cross flow processes 7 UsingtheHolder 7 1 Introduction 42 Learning...

Page 43: ...elationship between your pump speed setting and the feed flow rate it may be difficult to consistently achieve the process conditions you desire Therefore to ensure consistent performance and ease of...

Page 44: ...validation orqualitycontrol Key operationaldataincludesthese Transmembrane pressure Feed pressure Retentatepressure Producttemperature Feed volume initialandfinal Diafiltrate volume Permeateflow rate...

Page 45: ...eofproductyoucanrecoverfromthe systempipingand cassette Themethodinvolvesslightlyoverconcentratingtheproduct then adding asmallvolumeof permeatebackintothesystemtoflushconcentratedproductfromthe feed...

Page 46: ...loop Stop thepump Repeatstep5asneededtorecoverthemaximumvolume ofproduct Retentate Pump Valve Pressure Feed gauge Feed reservoir Kvick Lab holder Collection vessel Permeate Drain line Collection vess...

Page 47: ...r Section Seepage 8 1 Introduction 48 8 2 Stage1 Cleaningacassetteafteruse 49 8 3 Stage2 Measuringwaterflux 50 8 4 Stage3 Storing thecassetteandholder 51 8 CleaningandStoring LearningtoUsetheKvickLabC...

Page 48: ...ovescontaminatesfromthe cassette Flushing with waterremovesthecleaningsolutionfromthecassette MeasuretheWaterFlux youmeasurethewaterfluxandcompareittothewaterfluxwhenthe cassettewasnew Youcanthen dete...

Page 49: ...nd pumpabout10percentofthecleaningsolutionthroughpermeatelineto waste 5 Stopthepumpandconfigurethesystemsothattheretentateandpermeatelines dischargeintothefeedreservoir Fullyopenthefeedandretentateval...

Page 50: ...ssureforyourtypeof cassette 0 7barg 10psig forultrafiltrationcassettes anddeterminethe cassette swaterflux normalized toawatertemperatureof20 C Interpreting water flux values Comparethepost cleaningfl...

Page 51: ...surethestoragesolutionfullycoversthecassette Placethe sealed bag ina protected locationatatemperatureof4 C Forshort termstorage fivetotendays keepthecassetteintheholderandcirculatethestorage solutionf...

Page 52: ...9 Troubleshooting In this chapter Section Seepage 9 1 Troubleshooting guide 53 9 Troubleshooting 52 LearningtoUsetheKvickLabCassetteHolderUserManual18117269AI...

Page 53: ...andbackingplatefor scratches Replaceorhave Cytivapolishthescratched surface Processsolutionleaksfrom sanitary connections Sanitaryconnectiongasket missing worn damaged improperlymounted orwrong size I...

Page 54: ...kwithhand orby applyingsoapywaterand lookingforbubbles Remove cassetteandgasketand inspectsealingsurfaceofflow manifold backingplate and cassette Faultyvalve Checkintegrityofvalvesand replaceasneeded...

Page 55: ...nbodilyinjuryfromtheaccidentalstartingof thepump orunexpectedreleaseofpressure Topreventbodily injury disconnectthepowercable disconnectthecompressedair line anddepressurizethesystembeforeperformingin...

Page 56: ...Inspectthe sanitaryconnectiongasketsfordamageorwearandreplacethegasketsasneeded Checktherigidityofthestand Ifthestandwobbles tightenthescrewsthatholdthelegsand crossbracestogether Ifthemating surface...

Page 57: ...nifoldchannels withcompressed air Wipetheholderdrywithaclean lint freecloth Tip The nut torque values are based on the tie rod threads being dry You should not oil the tie rod threads Oiled tie rod th...

Page 58: ...ifoldandthebacking plate Installthespacers washers andnuts andlightlyhandtightento hold thecloth and backingplateinplace Placetheholderinadry protectedareasuchasacabinetto preventcontaminationoraccide...

Page 59: ...ications In this chapter Section Seepage 11 1 Sizeand dimensions 60 11 2 Materialsofconstruction 61 11 3 Operating parameters 62 11 Specifications LearningtoUsetheKvickLabCassetteHolderUserManual18117...

Page 60: ...ortfittings 1 2in TC Permeateportfittings 1 2in TC Dimensions approximateWxD xH 23x25x19cm 9x10x7 5in Shipping weight approximate 7 7kg 17lb 11 Specifications 11 1 Sizeanddimensions 60 LearningtoUseth...

Page 61: ...anifold Polished316Lstainlesssteel Ra 0 8um Backplate Polished316Lstainlesssteel Nonwetted parts Tie rods washers stand Stainlesssteel Tie rod nuts Bronze 11 Specifications 11 2 Materialsofconstructio...

Page 62: ...m2 1 2ft2 cassette Recommended operating temperature 5to50 C Maximumoperating temperature 121 C 250 F Maximumoperating pressure 4barg 60psig OperatingpH 1to14 Cassetteholdupvolume 20m Hardwareholdup v...

Page 63: ...pendix In this chapter Section Seepage 12 1 Chemicalcompatibility 64 12 2 Cassette sanitizingAgents 65 12 3 Partsandaccessories 66 12 Appendix LearningtoUsetheKvickLabCassetteHolderUserManual18117269A...

Page 64: ...sistant Ethyleneglycol50 Resistant Resistant Formaldehyde1 7M Resistant Resistant Formicacid10 Resistant Resistant Glycerol100 Resistant Resistant Hydrochloricacid 0 1M Notrecommended Resistant Isopro...

Page 65: ...zing Agents SanitizingAgent SanitizingConditions 0 5to1Nsodiumhydroxide Minimumcontacttimeforsanitization 60minutes 12 Appendix 12 2 CassettesanitizingAgents LearningtoUsetheKvickLabCassetteHolderUser...

Page 66: ...KvickLabholdertorquewrench KLTW0001 56 4112 84 1KvickLabcassettegasket KYLAGS001011 56 4113 66 3KvickLabcassettegaskets KYLAGS001033 56 4113 65 inchTC CPM stylefitting KLSY0105PCM05 56 4113 92 CPM fit...

Page 67: ...D Descriptionofholder 19 Documentation 7 E Expertadvice 24 43 45 57 nooilontierodthreads 57 Notrunningpumpsdry 45 preparingapumpcurve 43 Tierodthreads nooil 24 torquingtierodnuts 24 F FeaturesofKavic...

Page 68: ...use 27 38 bufferconditioning 38 Processflow 19 41 Productrecovery 45 ProperuseofKvickLabsystem 8 Pumpdryrunning damage 10 R Recoveringproduct 45 Recyclinginformation 4 disposalofelectricalcomponents 4...

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Page 70: ...sandPlasticCompanyInc Allotherthird partytrademarksarethepropertyoftheirrespectiveowners 2020 2021Cytiva Allgoodsandservicesaresoldsubjecttothetermsandconditionsofsaleofthesupplyingcompanyoperatingwit...