Check the integrity of the Cassette
When you establish that the system is leak-free, test the integrity of the Kvick Lab cassette by
following these steps.
Step
Action
1
Ensure the compressed air valve is closed and the pressure regulator is set to 0 barg (0
psig). Close the feed and drain valves, and open the retentate and permeate valves.
2
Remove water from the cassette feed-retentate path by opening the compressed air
valve, and slowly adjusting the pressure regulator to 0.3 barg (5 psig). Flow air through
the system until water stops draining from the retentate line. Close the retentate valve
to allow the air pressure to drive the water from the permeate port and permeate line.
3
Slowly adjust the pressure regulator to 1 barg (15 psig).
4
Fill a 50-ml graduated cylinder with water and place it inverted in a 500-ml beaker half
filled with water. Connect flexible tubing to the permeate outlet (
).
5
When the air bubble rate is stable, note the time and the standing air volume in the
graduated cylinder. When 5 to 10 ml of air has collected in the graduate cylinder, record
the time and air volume again.
6
Using the time and air volume data, calculate the air diffusion rate in ml/min/ft
2
@ 1 barg
(15 psig).
7
Compare your results with the specifications below. If your measured flow rate is less
than the specification, the Kvick Lab cassette is acceptable for use. The specifications
are:
60 ml/min/Kvick Lab cassette at 1 barg for 10K and 10KS cassettes
60 ml/min/Kvick Lab cassette at 1 barg for 30K cassettes
60 ml/min/Kvick Lab cassette at 1 barg for 50K cassettes
60 ml/min/Kvick Lab cassette at 1 barg for 100K cassettes
8
When the Kvick Lab cassette passes integrity testing, you can sanitize it (Stage 4),
condition it with buffer (Stage 5), or process product as required.
9
If the Kvick Lab cassette fails the integrity test, contact your Cytiva representative for
help.
6 Preparing a Cassette for Use
6.4 Stage 3 – Test the integrity of the cassette
36
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI
