6.5 Stage 4 – Sanitizing the Kvick Lab cassette
Clean and rinse the Kvick Lab cassette, then follow these steps to sanitize the cassette:
Step
Action
1
Configure the system so that the retentate and permeate lines discharge into the feed
reservoir (
). Close the drain valve.
2
Fill the feed reservoir with 1.5 to 2 liters of 0.1 N sodium hydroxide for each cassette in
your system. The temperature of the solution should be 35 to 45 °C.
3
Open the feed, retentate, and permeate valves. Start the pump. Adjust the retentate
valve and pump speed to obtain a retentate pressure of 0.7 barg (10 psig) and the
following retentate flow rate:
875 ml/min for each 0.11 m
2
(1.2 ft
2
) Kvick Lab cassette installed
4
Circulate the sanitizing solution for minimum of 60 minutes at a temperature of 35 to 45
°C. Stop the pump. If you are going to store the Kvick Lab cassette, rinse and add storage
solution as described in
Chapter 11 Specifications, on page 59
5
If you are sanitizing the Kvick Lab cassette before use, drain the sanitizing solution from
the system and then rinse the Kvick Lab cassette following the rinsing procedure in
Section 5.1 Introduction, on page 22
Kvick Lab
cassette
Pump
Valve
Pressure
Retentate
Feed
gauge
Feed reservoir
Permeate
Drain
line
Figure 6.5: Typical setup for sanitizing a Kvick Lab cassette and system
6 Preparing a Cassette for Use
6.5 Stage 4 – Sanitizing the Kvick Lab cassette
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI
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