8.2 Stage 1 – Cleaning a cassette after use
You can reuse a cassette if you clean and store it properly. To clean a used cassette, flush it with
buffer, WFI, or DI water as described in
Section 6.2 Stage 1 – Rinse storage solution from the cassette,
. Then clean the cassette following these steps:
Step
Action
1
Configure the system so that the retentate and permeate lines discharge to waste (
). Close the feed, retentate, and permeate valves.
2
Prepare the appropriate volume of a cleaning solution (1.5 to 2 liters per 0.11 m
2
(1.2 ft
2
)
Kvick Lab cassette. The Table below describes recommended cleaning solutions.
Tip:
Heating the rinse water or buffer to 40 to 50 °C can increase the effectiveness of the
rising process.
3
Fill the feed reservoir with the cleaning solution.
4
Open the feed and retentate valves and pump about 10 percent of the cleaning solution
through the retentate line to waste. Open the permeate valve and close the retentate
valve and pump about 10 percent of the cleaning solution through permeate line to
waste.
5
Stop the pump and configure the system so that the retentate and permeate lines
discharge into the feed reservoir. Fully open the feed and retentate valves. Crack open
the permeate valve.
6
Start the pump. Adjust the retentate valve and pump speed to obtain 1 to 1.5 times your
processing crossflow rate.
7
Circulate the cleaning solution for 30 to 60 minutes.
8
Drain the cleaning solution from the system using the drain line.
9
Rinse the cassette and system with clean water as described in
Rinse storage solution from the cassette, on page 28
Table 8.1: Recommended cleaning solutions
Cleaning Agent
Cleaning Conditions
0.5 M NaOH
30 minutes at room temperature
1 M NaOH
2 hours at 50 °C
0.5 M NaOH with 300 ppm NaOCl
30 minutes at room temperature
0.5 M NaOH with 500 ppm NaOCl
30 minutes at room temperature
0.5 M NaOH, then 0.5 M H
2
SO
4
30 minutes at room temperature for each solution
Double cleaning with 0.5 M NaOH
with 300 ppm NaOCl
30 minutes at room temperature for each cycle
8 Cleaning and Storing
8.2 Stage 1 – Cleaning a cassette after use
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI
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