8.4 Stage 3 – Storing the cassette and holder
Short-term storage
After completing stages 1 and 2, flush the cassette with a storage agent as described below to keep
the membrane wet and minimize biological growth:
•
For storage less than 3 days, use 0.2 M filtered water
•
For storage less than 6 months, use 0.1 N NaOH
•
For storage longer than 6 months, use a storage solution of 0.1 N NaOH and store the cassette at
4 °C.
For long-term storage (more than ten days), remove the cassette from the holder and place it into an
air-tight plastic tub or heavy-duty, zip-lock-type plastic bag. Add about 50 to 100 ml of the storage
solution to the plastic bag and seal it. Ensure the storage solution fully covers the cassette. Place the
sealed bag in a protected location at a temperature of 4 °C.
For short-term storage (five to ten days), keep the cassette in the holder and circulate the storage
solution for 10 to 15 minutes, close the system valves, disconnect the electrical power to the feed
pump, and ensure the feed reservoir is properly sealed. On manually-clamped holders, loosen the tie
rod nuts alternately ½ turn at a time until you can just turn the nuts by hand. Tighten the nuts to a
torque of 10 to 15 Nm (88 to 133 in-lbs). On hydraulic systems, keep the cassettes and gasket in the
holder and pressurize the hydraulic system to 35 to 69 barg (500 to 1000 psig) to prevent fluid from
leaking from the cassette holder.
8 Cleaning and Storing
8.4 Stage 3 – Storing the cassette and holder
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